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Detection of Gene Amplification in Human Breast Tumors

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
29230
Program Year/Program:
1995 / SBIR
Agency Tracking Number:
29230
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
AMPLICON CORPORATION
266 E PULASKI RD GREENLAWN, NY 11740
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1995
Title: Detection of Gene Amplification in Human Breast Tumors
Agency: HHS
Contract: 1 R43 CA67565-01,
Award Amount: $99,975.00
 

Abstract:

Cytogenetic studies indicate the presence of novel amplified DNAs specific for human breast cancersMYC, neu and PRAD1 have been found amplified in 5-40% of breast cancer biopsies and cell lines, andare amplified less frequently. Laborious approaches to clone DNA from the cytogenetic manifestationsamplification have not proven to be efficient or simple means to novel oncogenes. RDA or representatanalysis is a newly developed molecular method that allows directed cloning of the molecular differeclosely related populations of DNA molecules. Amplified DNA from breast and melanoma cancer cell linby RDA and two unique RDA probe sets for tumor analysis have been recovered. This Phase I study willfollowing issues: a) do current probes arise from novel genetic map locations and do these encode reoncogenes?; b) how can the RDA methods be optimized for cloning amplified DNA from human tumors, andDNAs detected by RDA probes amplified frequently in uncultured human tumor cells, and does this amplwith clinical parameters? In Phase II the informative probes will be clinically cataloged and the cofor sites amplified in human tumors will be expanded.

Principal Investigator:

Scott Powers
51661

Business Contact:

Small Business Information at Submission:

Amplicon Corp.
110 Lake Avenue S, Suite 35 Nesconset, NY 11767

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No