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Mulitplexed protein measurement by real-time immuno-PCR

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
70878
Program Year/Program:
2004 / SBIR
Agency Tracking Number:
HG003508
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
AMPLIFIED PROTEOMICS, INC.
AMPLIFIED PROTEOMICS, INC. 2462 WYANDOTTE ST MOUNTAIN VIEW, CA 94043 2313
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2004
Title: Mulitplexed protein measurement by real-time immuno-PCR
Agency: HHS
Contract: 1R43HG003508-01
Award Amount: $100,000.00
 

Abstract:

DESCRIPTION (provided by applicant): The real-time polymerase chain reaction (PCR) is the most commonly used method for quantitative gene expression profiling; it allows extremely sensitive measurement of hundreds of mRNA transcripts in parallel. We propose a method by which protein abundances can be quantitated using the same technology. This is achieved by implementing a sandwich immuno-assay format in which the detection antibodies are labeled with different DNA sequences. Real-time PCR is then used to quantify all of the DNA labels in parallel, thus revealing the quantities of the cognate proteins in the biological samples. Preliminary experiments show that real-time PCR using such antibody-DNA conjugates gives over two orders of magnitude more sensitive detection and almost three orders of magnitude increased dynamic range in comparison to standard ELISA, and standard deviations are very low (CV approximately 5%). Initial evidence supporting the ability to multiplex this method has also been obtained.

Principal Investigator:

David S. Wilson
5104320489
DSCOWILSON@YAHOO.COM

Business Contact:

Jack Zhai
5108665515
JYZHAI@YAHOO.COM
Small Business Information at Submission:

QUALITY PROTEOMICS, LLC
QUALITY PROTEOMICS, LLC 2462 WYANDOTTE ST MOUNTAIN VIEW, CA 94043

EIN/Tax ID: 870717457
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No