This goal is to construct a low-cost, polymeric, nanomaterial-based assay for the detection of multiple biological agents in a single device using our nanomanipulation technology to meet or exceed the sensitivity of the current two-line assays used in DoD applications. The nanodevice will exhibit… More

We propose to continue the development of rapid, handheld multiplexed assays for the detection of biological warfare agents and an associated handheld automatic reader. We will rigorously evaluate the 5-target assay developed in Phase I, and develop an 8-target multiplex during this Phase II… More

We propose to develop a universal strategy to encapsulate protein-based therapeutics using our patented non-toxic, non-immunogenic, water soluble, nanometer size capsules. These nanocapsules have a functionalized surface rendering them "unseen" by the normal immunogenic scavenging response of the… More

We propose to develop a multiplexed immunoassay for the detection of 2 - 4 medically relevant biomarkers of traumatic brain injury for use by front-line medical personnel and their civilian equivalents. The assay will provide results in less than 30 minutes, and provide information as to the actual… More

We propose to continue the development and characterization of a polymeric nanoencapsulation technology that can increase the lifetime of a protein therapeutic agent within a biological system. The materials have been demonstrated to exhibit no cytotoxicity and to efficiently deliver antibody… More

We propose that a comparative colorimetric assay be developed that will use a ticket of the same dimensions as the current ANP immunoassay tickets. This will allow the AChE test to be conducted using a similar workflow as the lateral flow immunoassay-based HHAs and be similarly read on the ANP… More

ANP Technologies proposes to develop a sample concentration system that uses commercial hollow fiber filtration technology with permanent anti-fouling coatings to concentrate biological contaminants in water sources or supplies to a level high enough to be readily detected. The filter technology… More

The feasibility of ANP's proposed seld-calibrated rapid test forf AChE inhibitors has been clearly shown in Phase I. Its compatability with ANP's handheld reader for immuno-assay based threat agent rapid testing has been confirmed. Phase II will finalize the design for robustness, full integration… More

ANP Technologies proposes to integrate a commercially available air sample concentrator from ICx MesoSystems with our automated detection instrument that uses the NIDSr multiplexed assays and NIDSr Auto-Reader (AR) device. A trigger device may also be incorporated into the final system, which might… More

A rapid assay for the detection of T2 trichothecene mycotoxin in water is proposed that will use ANP Technology, Inc.'s established handheld reader and rapid acetylcholinesterase (AChE) inhibitor test ticket format. Dipain-II and other candidate Dipain derivatives will be immobilized on cellulose… More