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ANTIVIRAL SCREENING AGAINST MULTIPLE VIRUSES

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
60464
Program Year/Program:
2002 / SBIR
Agency Tracking Number:
AI051754
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
APATH, LLC
APATH, LLC 893 N. WARSON RD. SAINT LOUIS, MO 63141
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2002
Title: ANTIVIRAL SCREENING AGAINST MULTIPLE VIRUSES
Agency: HHS
Contract: 1R43AI051754-01
Award Amount: $100,000.00
 

Abstract:

DESCRIPTION (Provided by applicant): Although cell-based screening has been used successfully throughout the drug-discovery field, it is problematic when screening for antiviral compounds. This is because it requires inoculation of infectious virus onto the cells and the production of additional infectious progeny virus. Handling such infectious material is not easily compatible with the high throughput process of screening large libraries of compounds. Partial viral replication systems offer a way around this problem. Viral genomes which lack one or more genetic elements that are essential for carrying out a complete replication cycle offer a system in which viral genomic replication and many other viral pathways occur within cells, but no infectious virus is produced. A screening process that utilizes these incomplete viral genomes can identify inhibitors of any biochemical pathway involved in viral genome replication. In this proposal we plan to expand the capabilities of this approach to be able to screen for multiple viruses simultaneously. By combining cell lines, each of which contain a partially replicating viral genome, we can screen for antiviral activity against each of the viruses simultaneously. In addition to measuring the effect of a compound on genomic replication of several viruses, this system provides information on the specificity of the antiviral effect. This information is helpful in accessing whether the effect is acting on a specific viral target or on a cellular target and thus exerting its effect on the virus(es) indirectly. In addition this approach allows for the identification of compounds which exhibit broad antiviral activity and could therefore be effective against more than one virus. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

Principal Investigator:

Paul D. Olivo
3148128144
OLIVO@APATH.COM

Business Contact:

L. Milton
3148128160
MILTON@APATH.COM
Small Business Information at Submission:

APATH, LLC
893 N. WARSON RD. ST. LOUIS, MO 63141

EIN/Tax ID: 431828778
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No