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INDICATOR CELLS FOR ANTIVIRAL SCREENING FOR FILOVIRUSES

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
60520
Program Year/Program:
2002 / SBIR
Agency Tracking Number:
AI052917
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
APATH, LLC
APATH, LLC 893 N. WARSON RD. SAINT LOUIS, MO 63141
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2002
Title: INDICATOR CELLS FOR ANTIVIRAL SCREENING FOR FILOVIRUSES
Agency: HHS
Contract: 1R43AI052917-01
Award Amount: $100,000.00
 

Abstract:

DESCRIPTION (provided by applicant): The goal of this proposal is to develop the commercial potential of a cell-based assay to screen for compounds with antiviral activity against filoviruses such as Ebola and Marburg viruses. The basis of this bioassay, which has been prototyped with respiratory syncytial virus, is infection-independent expression of a reporter gene from an artificial viral genome present within the cytoplasm of transformed cells. Filoviruses include some of the most notorious human pathogens and cause significant morbidity and mortality due to severe hemorrhagic fever syndromes. Unfortunately, very few therapeutic agents are available to treat infections with these viruses. Efforts to find new antiviral agents will require methods that are amenable to highthroughput assays. Since all viruses are obligate intracellular microbes, any bioassay that determines the effect of various agents on viral replication, must be based on cell culture. This involves working with infectious virus, which is obviously not feasible for agents such as EBOV and MBGV. Significant advances have been made in identifying the cis-acting elements and trans-acting factors involved in replication of many pathogenic negative-strand RNA viruses, including these filoviruses. We plan to develop infection-independent Ebola and Marburg virus minigenome-expression systems in which the expression of an easily measurable enzyme is dependent on the RNA replication and transcription factors that are essential for viral replication. We plan to use these systems in cell-based assays for screening antiviral compounds.

Principal Investigator:

Paul D. Olivo
3148128144
OLIVO@APATH.COM

Business Contact:

L. Milton
3148128160
MILTON@APATH.COM
Small Business Information at Submission:

APATH, LLC
893 N. WARSON RD. ST. LOUIS, MO 63141

EIN/Tax ID: 431828778
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No