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ELECTRODE ARRAYS IN TISSUE CULTURE

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
16789
Program Year/Program:
1993 / SBIR
Agency Tracking Number:
16789
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
APPLIED BIOPHYSICS, INC.
APPLIED BIOPHYSICS, INC. 185 Jordan Road TROY, NY 12180
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1993
Title: ELECTRODE ARRAYS IN TISSUE CULTURE
Agency: HHS
Contract: N/A
Award Amount: $486,623.00
 

Abstract:

THE GOAL OF THIS PROJECT IS TO DEVELOP AN ARRAY OF 10-(4)CM(2) ELECTRODES FOR USE WITH A NEW INSTRUMENTAL METHOD FOR TISSUE CULTURE. THE METHOD, REFERRED TO AS ECIS (ELECTRIC CELL-SUBSTRATE IMPEDANCE SENSOR), IS A NON-INVASIVE TECHNIQUE TO MEASURE SEVERAL IN VITRO CELL PARAMETERS THAT HAVE PREVIOUSLY BEEN DIFFICULT TO QUANTIFY. THESE INCLUDE CELL MOTILITY, ADHESION AND SPREADING, MORPHOLOGY, AND THE BARRIER FUNCTIONS OF CELL LAYERS. THE ELECTRONICS AND SOFTWARE OF PROTOTYPE INSTRUMENT HAVE BEEN WORKED OUT. TO BE SUCCESSFUL, HOWEVER, A PRACTICAL METHOD OF FABRICATING ELECTRODES IS NOW REQUIRED. IN PHASE I, RESEARCH WILL BE CARRIED OUT TO TEST THE FEASIBILITY OF USING MODERN PHOTORESIST TECHNOLOGY TO PRODUCE ARRAYS OF GOLD ELECTRODES. SPECIFICALLY, STUDIES WILL ADDRESS (1) MODIFICATION OF PHOTORESIST TECHNOLOGY FOR PLASTIC AND GLASSSUBSTRATES, (2) STABILITY OF PHOTORESISTS IN CULTURE MEDIA, AND (3) COMPATIBILITY OF THE SYSTEM FOR CELL CULTURE. IF THESE STUDIES ARE SUCCESSFUL, A FINAL PROTOTYPE WILL BE ASSEMBLED AND TESTED, WHERE COMPUTER-ADDRESSABLE ELECTRODE PAIRS WILL BE LOCATED ON THE FLOOR OF EACH WELL OF A STANDARD TISSUE CULTURE MULTIWELL PLATE. THE LONG TERM OBJECTIVE OF THIS WORK IS TO DEVELOP THE ECIS INSTRUMENT INCORPORATING ELECTRODE ARRAYS FABRICATED USING STATE-OF-THE ART MANUFACTURING PROCEDURES. THE METHOD WILL THEN BE A PRACTICAL AND HIGHLY SENSITIVE MEANS TO MONITOR CELL BEHAVIOR FOR BOTH MEDICAL AND BIOLOGICAL RESEARCH. THE GOAL OF THIS PROJECT IS TO DEVELOP AN ARRAY OF 10-(4)CM(2) ELECTRODES FOR USE WITH A NEW INSTRUMENTAL METHOD FOR TISSUE CULTURE. THE METHOD, REFERRED TO AS ECIS (ELECTRIC CELL-SUBSTRATE IMPEDANCE SENSOR), IS A NON-INVASIVE TECHNIQUE TO MEASURE SEVERAL IN VITRO CELL PARAMETERS THAT HAVE PREVIOUSLY BEEN DIFFICULT TO QUANTIFY. THESE INCLUDE CELL MOTILITY, ADHESION AND SPREADING, MORPHOLOGY, AND THE BARRIER FUNCTIONS OF CELL LAYERS. THE ELECTRONICS AND SOFTWARE OF PROTOTYPE INSTRUMENT HAVE BEEN WORKED OUT. TO BE SUCCESSFUL, HOWEVER, A PRACTICAL METHOD OF FABRICATING ELECTRODES IS NOW REQUIRED. IN PHASE I, RESEARCH WILL BE CARRIED OUT TO TEST THE FEASIBILITY OF USING MODERN PHOTORESIST TECHNOLOGY TO PRODUCE ARRAYS OF GOLD ELECTRODES. SPECIFICALLY, STUDIES WILL ADDRESS (1) MODIFICATION OF PHOTORESIST TECHNOLOGY FOR PLASTIC AND GLASSSUBSTRATES, (2) STABILITY OF PHOTORESISTS IN CULTURE MEDIA, AND (3) COMPATIBILITY OF THE SYSTEM FOR CELL CULTURE. IF THESE STUDIES ARE SUCCESSFUL, A FINAL PROTOTYPE WILL BE ASSEMBLED AND TESTED, WHERE COMPUTER-ADDRESSABLE ELECTRODE PAIRS WILL BE LOCATED ON THE FLOOR OF EACH WELL OF A STANDARD TISSUE CULTURE MULTIWELL PLATE. THE LONG TERM OBJECTIVE OF THIS WORK IS TO DEVELOP THE ECIS INSTRUMENT INCORPORATING ELECTRODE ARRAYS FABRICATED USING STATE-OF-THE ART MANUFACTURING PROCEDURES. THE METHOD WILL THEN BE A PRACTICAL AND HIGHLY SENSITIVE MEANS TO MONITOR CELL BEHAVIOR FOR BOTH MEDICAL AND BIOLOGICAL RESEARCH.

Principal Investigator:

Charles Keese
5182766438

Business Contact:

Small Business Information at Submission:

Applied Biophysics Inc.
2080 Van Antwerp Rd Schenectady, NY 12309

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No