USA flag logo/image

An Official Website of the United States Government

NOVEL GELS FOR RAPID RESOLUTION OF HIGH MOLECULAR WEIGHT DNA

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
19288
Program Year/Program:
1994 / SBIR
Agency Tracking Number:
19288
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
APPLIED BIOTECH CONCEPTS, INC.
25 SCIENCE PARK NEW HAVEN, CT 06511
View profile »
Woman-Owned: No
Minority-Owned: Yes
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1994
Title: NOVEL GELS FOR RAPID RESOLUTION OF HIGH MOLECULAR WEIGHT DNA
Agency: HHS
Contract: N/A
Award Amount: $526,237.00
 

Abstract:

MAPPING OF GENOMIC DNA WILL REQUIRE THE SEPARATION OF VERY LARGE DNA MOLECULES (CA. 0.1 TO 3000 MEGABASE-PAIRS (MBP)). WHILE SMALLER DNA MOLECULES (0.1-20 KILOBASE-PAIRS (KBP)) ARE EFFICIENTLY AND RAPIDLY RESOLVED BY THE CONVENTIONAL CONSTANT FIELD GEL ELECTROPHORESIS (CFGE), THE RESOLUTION OF DNA SIZES IN THE RANGE OF 0.1 TO 10 MBP HAS BEEN ACHIEVED ONLY USING VARIANTS OF PULSED-FIELD GEL ELECTROPHORESIS (PFGE) IN AGAROSE GELS. THE LONG TIME SPAN (1-3 WEEKS) NEEDED OR PFGE AND THE LONG TOTAL MIGRATION DISTANCE IN THE GEL RESULT IN SOME BREAKDOWN OF DNA. NEW GENE MAPPING TECHNIQUES ARE BEING DEVELOPED WHICH REQUIRE RESOLUTION OF COSMIDS IN THE SIZE RANGE OF 30 TO 100 KBP. THESE AND FURTHER DEVELOPMENTS IN RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP) AND COARSE GENE MAPPING ANALYSES WILL REQUIRE SIMPLE AND RAPID RESOLUTION OF DNA MOLECULES IN THE SIZE RANGE OF 50 KBP AND LARGER. WE HAVE DESCRIBED THE SYNTHESIS AND USE OF NEW PROPRIETARY GELS WHICH ARE EXPECTED TO PROVIDE RAPID AND EFFICIENT ELECTROPHORETIC RESOLUTION OF SUCH LARGE DNA MOLECULES. THESE GELS ARE EXPECTED TO EXTEND THE RESOLVING SIZE RANGE OF CFGE AT LEAST UPTO 0.5-1 MBP AND MAY BE ABLE TO PROVIDE PERFORMANCE ADVANTAGES WITH PFGE AS WELL.

Principal Investigator:

Anil Diwan
2034559378

Business Contact:

Small Business Information at Submission:

Applied Biotech Concepts Inc.
25 Science Park New Haven, CT 06511

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No