Applied genetics laboratories, inc. proposes to utilize newly developed molecular genetic techniques to analyze the spectrum of mutagenic events that occur at the tk and hprt loci in mouse l5178y cells. the purpose of these studies isto determine if the spectrum of genetic abnormalities registered… More

Recently developed molecular techniques will be used to analyze mutations at the tk locus in mouse l5178y cells exposed to chemical mutagens. because mutation assays measure phenotype, not genotype, scored mutations could result from other mechanisms. sequence analysis will determine whether the… More

We have described a new technology called "preparative in situ hybridization (Prep-I.S.H.), which is capable of isolating a set of cDNA or genomic clones according to their chromosome location. The technology takes advantage of in situ hybridization of cloned DNAs to metaphase chromosome spreads,… More

Our research decribes a strategy for producing a high density set of PCR-base microsatellite markerpolymorphic in L5178Y, the cell line used in the mouse lymphoma assay (MLA). These polymorphic marketwo detailed long range restriction maps in the vicinity of the thymidine kinase (tk-1) gene, with… More

This proposal describes a set of reagents which will make gene mapping to chromosomes and chromosomas well as gene finding exercises, accessible to any molecular biologist or geneticist familiar withtechniques. These reagents, called the Gene. ID system, are a set of "dot-blots". The… More

We propose using new molecular techniques to survey the spectrum of mutagenic events occurring at tL5178Y cells. We will compare this spectrum with the range of abnormalities occurring in human cancemouse lymphoma assy (MLA) as an accurate reflection of events of human significance. … More

Fluorescent chromosome painting probes allow identification of a variety of chromosome abnormalitieinterphase and mitotic cells. Mouse cytogenetics, handicapped by difficult chromosome identificationchromosome paints in many research and commercial applications, including in vivo and in vitro… More

This proposal describes a method for preparing cDNA sublibraries specific to certain chromosome regprocedure, which we call selection of hybrids by affinity capture (SHAC), can be described in two stbanded mitotic chromosomes are microdissected and PCR- amplified using a universal degenerate… More

APPLICANT?S DESCRIPTION: This proposal describes technical approaches for efficient and high throughput production of region specific genomic and cDNA microarrays with the ultimate goal of deciphering cancer-associated genes involved in various human neoplasias. The techniques will be applied to… More