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Polymorphic Markers for Chromosome Band 11e2

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
29423
Program Year/Program:
1995 / SBIR
Agency Tracking Number:
29423
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
APPLIED GENETICS LABORATORIES
1335 GATEWAY DR, STE 2001 MELBOURNE, FL 32904
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1995
Title: Polymorphic Markers for Chromosome Band 11e2
Agency: HHS
Contract: 1 N43 ES51001-000,
Award Amount: $99,980.00
 

Abstract:

Our research decribes a strategy for producing a high density set of PCR-base microsatellite markerpolymorphic in L5178Y, the cell line used in the mouse lymphoma assay (MLA). These polymorphic marketwo detailed long range restriction maps in the vicinity of the thymidine kinase (tk-1) gene, with tdefined distances from each other and the tk-1 gene. The first, lower resolution map will define thethe tk-1 gene, and will contain a PCR-based polymorphic marker approximately every 1 Mb. Multiplex Pbe identified so that mutants can be assayed through the entire 10 MB regon in a single reaction, atThe second, higher resolution map will contain apporoximately 10 PCR-based polymorphic markers, posikb, in the 1 Mb region in the immediate vicinity of the tk-1 gene. Multiplex PCR conditions will alssecond set of markers so that mutants can be analyzed in this 1 Mb region at a resolution of 100 kb.these polymorphic markers, we will construct a genomic library of mouse chromosome band 11E2, accordfollowing strategy: Band 11E2 will be microdissected form normal metaphase spreads, PCR amplifies usoligonucleotide primer, and cloned. The library will be screened with a microsatellite repeat probe,(CA)n repeats will be sequenced. PCR primer sets flanking the repeats will be designed and synthesizbe assayed for heterozygosily. Polymorphis (CA)n repeats will be identifies by the production of two

Principal Investigator:

Lisa Davis
30568

Business Contact:

Small Business Information at Submission:

Applied Genetics
1335 Gateway Drive, Suite 2001 Melbourne, FL 32904

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No