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Preparation of Mouse Chromosome Painting Kits

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
29424
Program Year/Program:
1997 / SBIR
Agency Tracking Number:
29424
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
APPLIED GENETICS LABORATORIES
1335 GATEWAY DR, STE 2001 MELBOURNE, FL 32904
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1997
Title: Preparation of Mouse Chromosome Painting Kits
Agency: HHS
Contract: 1 N43 ES51002-000,
Award Amount: $676,404.00
 

Abstract:

Fluorescent chromosome painting probes allow identification of a variety of chromosome abnormalitieinterphase and mitotic cells. Mouse cytogenetics, handicapped by difficult chromosome identificationchromosome paints in many research and commercial applications, including in vivo and in vitro genteWidespread commerical use of mouse painting probes requires reliability and ease of use in laboratorexperienced in mouse cytogenetics. Reliability depends on blocking probe hybridization to genomic reEase of use and versatility can be enhanced by simplifying fluorescence detection. This research proprobes using reliable mouse Cot1 repetitive DNA produced in house, increasing consistency of the finreducing pre-annealing time and cost. Alternatively, repeat sequences may be remove form painting prof repetitive sequences to immobilized repetitive DNA: resultant probe will be tested for painting.increase probe specificity and reduce the need for Cot1 pre-annealing. Labeling with different conjuallow users to combine probes and distinguish them by fluorescence. Direct labeling with fluorochromnucleotides may eliminate post-hybridization detection steps. Finally, the feasiblity of preparing frather than paint specific chromosomes for interphase chromosome counting and mapping will be examinbacterial artificail chromosome libraries for sequences mapping will be wexamined by screening bactechromosome libraries for sequences mapping to specific chromosome ends.

Principal Investigator:

Melissa Liechty
30568

Business Contact:

Small Business Information at Submission:

Applied Genetics
1335 Gateway Drive, Suite 2001 Melbourne, FL 32904

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No