USA flag logo/image

An Official Website of the United States Government

DEVELOPMENT OF CANCER REGION SPECIFIC MICROARRAYS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
53827
Program Year/Program:
2001 / SBIR
Agency Tracking Number:
1R43CA089978-01
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
APPLIED GENETICS LABORATORIES
1335 GATEWAY DR, STE 2001 MELBOURNE, FL 32904
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2001
Title: DEVELOPMENT OF CANCER REGION SPECIFIC MICROARRAYS
Agency: HHS
Contract: N/A
Award Amount: $99,885.00
 

Abstract:

APPLICANT?S DESCRIPTION: This proposal describes technical approaches for efficient and high throughput production of region specific genomic and cDNA microarrays with the ultimate goal of deciphering cancer-associated genes involved in various human neoplasias. The techniques will be applied to the human 5q31-qter region for feasibility studies. This region and its homologous region in mouse have been shown to be critical in mouse and human hepatocellular carcinomas. We will take two independent approaches to reach the goal of identifying genomic regions harboring genetic alterations and the genes encoded by them. For production of genomic microarrays, we will isolate YAC and BAC contigs in the 5q31-qter region and amplify by inter-Alu primers to obtain a fair representation of this region. We will microarray these amplicons and screen with probes obtained from normal and cancerous tissues (each with different fluorochromes). The ratio of fluorescent intensities will be used to identify amplified and deleted regions of the genome. For development of region-specific cDNA arrays, we ill make use of genomic draft sequencing information to select all coding sequences in the 5q31-qter region. We will array these enriched cDNAs and screen them with cDNA probes derived from normal and cancerous tissues. The high throughput production of genomic and cDNA microarrays will facilitate detailed genetic and transcription mapping of cancer associated regions of the human genome. PROPOSED COMMERCIAL APPLICATION: Commercially available region specific microarrays will be offered to researchers who concentrate on the regions of genome that are critical in human neoplasias. Therefore, the ultimate goal of this project is to generate commercially available region specific, custom made genomic and cDNA microarrays that can be screened with fluorescently labeled genomic and cDNA probes to facilitate gene mapping and discovery efforts.

Principal Investigator:

John C. Hozier

Business Contact:


4077682048
JH@APPLIEDGENETICS.COM
Small Business Information at Submission:

APPLIED GENETICS LABORATORIES
1335 GATEWAY DR, STE 2001 MELBOURNE, FL 32904

EIN/Tax ID: 592421804
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No