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DEVELOPMENT OF PROTEASE-DEFICIENT, GRAM-POSITIVE HOST

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4685
Program Year/Program:
1987 / SBIR
Agency Tracking Number:
4685
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Applied Microbiology Inc
5 Brooklyn Navy Yard Brooklyn, NY 11205
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1987
Title: DEVELOPMENT OF PROTEASE-DEFICIENT, GRAM-POSITIVE HOST
Agency: HHS
Contract: N/A
Award Amount: $252,000.00
 

Abstract:

OUR LONG-TERM AIM IS TO DEVELOP A GRAM-POSITIVE ORGANISM FORTHE EXPRESSION AND SECRETION OF MEDICALLY AND INDUSTRIALLY USEFUL PROTEINS. ONE MAJOR OBSTACLE TO THE SUCCESSFUL DEVELOPMENT OF SUCH A SYSTEM IS THE PROTEOLYTIC DEGRADATION OF SECRETED PRODUCTS. SITE-DIRECTED DELETION MUTATIONS CONSTRUCTED IN VITRO HAVE ELIMINATED THE TWO MAJOR EXTRACELLULAR PROTEASES OF B. SUBTILIS. ALTHOUGH SIGNIFICANTLY DIMINISHED, PROTEOLYTIC DEGRADATION OF SECRETED PRODUCTS BY RESIDUAL PROTEASE REMAINS PROBLEMATIC. CHARACTERIZATION OF RESIDUAL PROTEASE ACTIVITY WILL BE PERFORMED PRELIMINARY TO CLONING AND SITE-DIRECTED DELETION OF ANY PROTEASE GENES IDENTIFIED IN THE PRESENT STUDY. MUTAGENESIS OF DOUBLE-PROTEASE-NEGATIVE STRAINS OF B. SUBTILIS, AND SCREENS OF OTHER BACILLUS SPECIES FOR PROTEASE-DEFICIENT STRAINS, WILL BE PERFORMED IN AN EFFORT TO DEVELOP A GRAM-POSITIVE HOST THAT DOES NOT DEGRADE SECRETORY PRODUCTS.

Principal Investigator:

Peter Blackburn
Principal Investigator
7188525353

Business Contact:

Small Business Information at Submission:

Applied Microbiology Inc.
#5 Brooklyn Navy Yard Brooklyn, NY 11205

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No