DOMINANT PEPTIDE INHIBITORS OF PLANT VIRUS REPLICATION
DESCRIPTION (Verbatim from Applicant's Abstract):We have developed a technology
for somatic cell genetics that leads rapidly to genetic agents that modulate
cellular phenotypes. The technology utilizes genetic assays in combination with
perturbagens, peptides that inhibit specific biochemical processes in cells,
causing a phenotypic shift. Our perturbagen technology is potentially able to
endow plants with novel phenotypes that would be very difficult to obtain by
conventional plant breeding.
The primary objective of the proposed research is to explore the use of
perturbagen methods to identify peptide aptamers that confer resistance to the
model viral pathogen tobacco etch virus (TEV), a member of the economically
important potyviruses. Phase I research involves:
(1)The identification of peptides that bind to TEV NIa and HC-Pro proteins and
TEV RNA genome using the technology developed at Arcaris (specifically,
perturbagen libraries) via modified yeast two-and three-hybrid systems.
(2)The expression of these peptides in cultured tobacco cells to identify
peptides that inhibit TEV replication. Protoplasts derived from these tobacco
cells will be infected with transcripts of TEV that has been modified to
include the b-glucuronidase (GUS) gene in its genome. The impact of these
peptides on TEV replication will be monitored by GUS activity.
PROPOSED COMMERCIAL APPLICATION:
In contrast to viral sequence-derived resistance, perturbagen technology circumvents
any potential risks associated with the expression of virus-derived transgenes. This
approach has the potential to engineer highly durable virus resistance in plants by
expression of peptides that interfere with the function several essential viral proteins.
In addition, this technology can be used to rapidly identify perturbagens with fungicidal,
bacteriocidal, and insecticidal properties that cna be expressed in plants.
Small Business Information at Submission:
Principal Investigator:Robert W. Sandrock
615 ARAPEEN DR, STE 300 SALT LAKE CITY, UT 84108
Number of Employees: