DESCRIPTION (provided by applicant): The purpose of this proposal is to develop a protein assay technology that will parallel the newly developing oligonucleotide microarray techniques. We will develop a peptide system capable of performing high throughput real time measurements in parallel on a… More

DESCRIPTION (provided by applicant): The purpose of this proposal is to develop a protein assay technology that will parallel the newly developing oligonucleotide microarray techniques. We will develop a peptide system capable of performing high throughput real time measurements in parallel on a… More

DESCRIPTION (provided by applicant): We propose the development of a microfluidic real-time PCR microarray technology that is far beyond the capability of today's hybridization-based DNA microarrays. This new technology will enable a broad range of research and diagnostic applications that require… More

DESCRIPTION (provided by applicant): The increasing use of DNA as a molecular tool has ushered the technology of de novo synthesis of DNA back to the center stage of modern biology. We propose the development of a robust, ultra high-throughput (UHT) technology capable of genome-scale production of… More

DESCRIPTION (provided by applicant): The long term goal of the proposed technology development is to take the current applications of the protein kinase (PK) substrate chips to the level suitable for proteome-scale applications and for developing clinical diagnostics. Recent years have witnessed… More

DESCRIPTION (provided by applicant): This is a SBIR Phase II application for developing microfluidic microchips containing 30k protein kinase (PK) substrate peptides for proteomic PK profiling of cell lysates. In recent years there has been a dramatic expa nsion in our understanding of PK (kinome)… More