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Parallel DNA Assembling on MicroChip

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
80368
Program Year/Program:
2006 / STTR
Agency Tracking Number:
HG003786
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
ATACTIC TECHNOLOGIES, INC.
2575 WEST BELLFORT SUITE 270 HOUSTON, TX 77054
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2006
Title: Parallel DNA Assembling on MicroChip
Agency: HHS
Contract: 1R41HG003786-01A1
Award Amount: $453,401.00
 

Abstract:

DESCRIPTION (provided by applicant): The increasing use of DNA as a molecular tool has ushered the technology of de novo synthesis of DNA back to the center stage of modern biology. We propose the development of a robust, ultra high-throughput (UHT) technology capable of genome-scale production of high fidelity synthetic DNA at about one percent of the cost and time of the current technologies. Our phase I plan will demonstrate the feasibility of the proposed method that includes parallel synthesis of an array of long DNA sequences on a capture microchip using annealing and ligation of assembling deoxyoligonucleotides (oligos). Our Phase I Specific Aims are: 1: Developing a method for parallel synthesis of an array of long DNA sequences on chip; 2: Developing monitoring and error correction methods essential for improving the efficiency and fidelity of the DNA synthesis. The proposed solid phase parallel synthesis technology has two significant advantages over the existing methods of DNA synthesis: (i) it has the potential to allow simultaneous assembling of thousands or more DNA sequences; (ii) it is a process that individual steps and individual synthesis can be monitored and optimized. If successful, one can expect a significant step forward in long DNA sequence preparation, similar to the advancement by moving oligonucleotide and peptide synthesis from solution to solid phase. A reliable, miniaturized, robust process will then replace the current slow, expensive, and still largely unpredictable gene synthesis processes.

Principal Investigator:

Xiaolian Gao
7137432805
XGAO@UH.EDU

Business Contact:

Xiaochuan Zhou
7136648189
XCZHOU@ATACTICTECH.COM
Small Business Information at Submission:

ATACTIC TECHNOLOGIES, INC.
2575 WEST BELLFORT SUITE 270 HOUSTON, TX 77054

EIN/Tax ID: 134246709
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
Research Institution Information:
UNIVERSITY OF HOUSTON
UNIVERSITY OF HOUSTON
4800 CALHOUN RD
HOUSTON, TX 77204
RI Type: Nonprofit college or university