Abstract:This project continues the investigation of Ultrasonic forces coupled with Field-Flow Fractionationseparate macromolecules and cells. A prototype Ultrasonic Field-Flow Fractionation apparatus has beefabricated and tested. The device is configurable in three modes. Retention has been demonstrated… more
Abstract:THE BLACK-LIP PEARL OYSTER, PINCTADA MARGARITIFERA, IS THE SOURCE OF RARE, VALUABLE BLACK PEARLS. CULTURE OF THIS SPECIES HAS PROVEN HIGHLY LUCRATIVE IN FRENCH POLYNESIA, WITH STRENGTHENING WORLDWIDE DEMAND. THE HAWAIIAN SUB-SPECIES, P. MARGARITIFERA GALTSOFFI, IS ON THE HAWAII STATE ENDANGERED… more
Abstract:NATIVE HAWAIIANS "FORM AN UNDERPRIVILEGED MINORITY BOTH IN HAWAII AND ON THE MAINLAND UNITED STATES, WITH CHARACTERISTICS SIMILAR TO NATIVE AMERICANS, BLACKS, AND HISPANICS" (ATU LIKE, 1989). THE NATIVE HAWAIIAN COMMUNITY HAS A HISTORY OF UNDERREPRESENTATION IN THE BUSINESS SECTOR. THE ULTIMATE… more
Abstract:MULTIPLE ACCESS PROTOCOLS ARE USED TO PERMIT LARGE NUMBERS OF INDEPENDENT TRANSMITTERS TO SHARE A SINGLE COMMUNICATION CHANNEL. THERE ARE THREE DIFFERENT TYPES OF MULTIPLE ACCESS PROTOCOLS WHICH ARE USED IN MOST WIRELESS DATA NETWORKS TODAY-TDMA, CDMA AND ALOHA. CDMA PROTOCOLS CAN BE EFFICIENT BUT… more
Abstract:Multiple access protocols are used to permit large numbers of independent transmitters to share a common communication channel. CDMA was developed as a multiple access network protocol for military applications during the last forty years. Recently considerable interest in using CDMA in the civilian… more
Abstract:THE OVERALL GOALS OF THIS PROJECT ARE TO DEVELOP IMMUNOASSAYS FOR THE DETECTION OF BRYOSTATIN 1 AND ITS ANALOGUES IN A VARIETY OF BIOLOGICAL MATRICES. THE BRYOSTATINS HAVE EXHIBITED STRONG ANTINEOPLASTIC ACTIVITY IN PRECLINICAL STUDIES AND ARE SCHEDULED TO ENTER PHASE I CLINICAL TRIALS LATER THIS… more
Abstract:Murine monoclonal antibodies which neutralize maitotoxin will be selected using a functionalassay that exploits the hemolytic action of maitotoxin. The resulting antibodies should be useful forseveral purposes. They will provide a means to measure the toxin content in crude extracts of fishtissues… more
Abstract:Dengue viruses are a leading cause of morbidity and mortality throughout the tropics andsubtropics. It is estimated that more than 100 million people in the tropics are infected annually by thisvirus. This threat has now reached the southern United States with the arrival of the Aedes… more
Abstract:Several fundamental parameters need to be addressed with regard to the development of biosensors. Protein stability is a major criteria for sensors devoted to long-term monitoring. The enzyme label is typically the most unstable componenet in biosensors. Additional problems encountered with… more
Abstract:The filamentous fungi, owing to their ability to secrete high levels of proteins, are attractive organisms for the expression and secretion of heterologous proteins of commercial and medical value. Secretion is a key element in their attraction, for secretion enables proper folding and processing of… more
Abstract:Natural testosterone and numerous synthetic analogs are known to stimulate biosynthesis ofproteins and are used as therapeutic agents. Most of them are DEA controlled substances. Improper usemay cause health risks. Steroid abuse is a widespread problem. There is a practical need to screen forabused… more
Abstract:High rate-of-fire weapons are subject to significant barrel erosion as projectile velocity and burst lengths are increased. Bore temperatures exceeding the melting point of steel can be easily reached because of the low thermal conductivity of the steel barrel. Barrels comprised of copper/graphite… more
Abstract:High-power ammonia arcjet thrusters are being investigated as primary propulsion systems for carrying satellites into high altitude orbits. As a method to enhance the power levels and efficiency of the engine, the thermal emissivity of the outer anode surface must be substantially enhanced. Ideally,… more
Abstract:In the next generation of air-breathing high-performance engines, significant engine performance improvements are envisioned by combusting hydrogen with air in a regeneratively cooled carbon/carbon composite combustion chamber. Surfaces subjected to aerodynamic heating will also be cooled by… more
Abstract:THIS PHASE I PROJECT IS DESIGNED TO TEST THE FEASIBILITY OF PURIFYING TAXOL BY IMMUNOAFFINITY CHROMATOGRAPHY. THE USE OF THIS TECHNIQUE FOR PURIFICATION OF A LOW MOLECULAR WEIGHT COMPOUND WITH LOW AQUEOUS SOLUBILITY HAS NOT BEEN REPORTED TO DATE. TAXOL-SPECIFIC MONOCLONAL ANTIBODIES WILL BE SELECTED… more
Abstract:A Taxol prodrug derivative will be prepared. This compound will be tested for its differential cytotoxicity compared to Taxol and for its ability to be enzymatically activated. The cytotoxicity of the prodrug in combination with a monoclonal-antibody enzyme conjugate in the presence of targeted and… more
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