Abstract:Although many fluorophores exist which are suitable for labeling biomolecules, most of them have absorbance and fluorescence wavelenghts which overlap with naturally occurring biological fluorescence. This complicates their use with optical bio- sensors. A very limited number of commercially… more
Abstract:We will develop an automated instrument for the detection and quantification of Giardia and Cryptoswater sources. The long-term objective is the development and commercialization of a portable, ruggeinstrument which rapidly processes filter concentrated samples without the use of trained… more
Abstract:In spite of the rapid advances in the speed and density of electronic components, a compact hardware package providing a complete real-time solution to vision problems remains beyond the capabilities of currently available technology. This project proposes to develop a smart CCD camera using a… more
Abstract:This proposed project combines ideas from neuro-biological modeling of the cochlea, engineering designs of optimal filters, the theoretical formulation using the calculus of variations, and optimal filtering and control. Several competing formulations will be developed. A potential breakthrough… more
Abstract:The goal of the proposed research is to recycle PET and eventually other plastic wastes, through depolymerization while avoiding costly purification and color-removal steps, into low-cost and highly durable rapid-setting rehabilitation materials. Successful implementation of the proposed technology… more
Abstract:MICROORGANISMS ARE PRIMARILY RESPONSIBLE FOR THE DEGRADATION OF TOXIC CHEMICALS THAT ENTER THE ENVIRONMENT. SUCCESSFUL ISOLATION AND EXPLOITATION OF THESE MICROBES FOR BIOREMEDIATION REQUIRES IDENTIFYING THE PHYSICO-CHEMICAL CONDITIONS UNDER WHICH DETOXICATION ACTIVITY IS MAXIMIZED. THIS WORK IS… more
Abstract:Our objective is to prove feasibility of ultrasensitive chemiluminescent assays for the 21 aminoacid polypeptide from HIV gp 120 by Western blotting and cystic fibrosis transmembrane regulator geneby Southern blotting. We have developed a proprietary enzyme-label chemiluminescent detection… more
Abstract:We will develop a new generation confocal microscope uniquely capable of ultraviolet (UV) as well as visible illumination and video-rate imaging. In Phase I, experiments will be conducted to determine the components and dimensions needed to correct for the longitudinal and lateral chromatic errors… more
Abstract:Optical microscopy is an important tool for investigations of biological structure and function inFar-field optics are restricted in resolution, however, to a physically defined limit of 200 nm. Invsubcellular organization and structure below this limit have required electron microscopy, which… more
Abstract:This Phase I proposal is aimed toward research and development of novel low cost commercially feasible environmentally-friendly biodegradable polymeric thermosetting materials based on epoxidized triglyceride oils. The inexpensive thermosets based on epoxidized soybean oil ($0.50/lb) and sucrose… more
Abstract:Nitrate contamination of ground water is a world wide problem. Nitrate at 10 ppm Nitrate-N (EPA's MCL) is toxic to infants, and chronic exposure may be harmful to adults. Physical and chemical nitrate removal methods only concentrate the pollutant, and bioremediation methods introduce other… more
Abstract:Measurement of stratospheric temperatures yields information critical to the monitoring of long-term atmospheric changes, weather forecasting, and scientific understanding of the middle atmosphere. We propose to develop a simple stratospheric temperature sounder, based on a Fabry-Perot… more
Abstract:Detailed models of limited aspects of vehicle performance are currently used in engineering development, e.g., DADDS, BRL-CAD/SQUASH, PRISM, etc. There is a need for an integrated model which analyzes the interactions among subsystems and evaluates performance of multiple system functions. Design… more
Abstract:X-RAY ABSORPTION SPECTROSCOPY (XAS) USING SYNCHROTRON RADIATION IS BEING DEVELOPED AS A UNIQUE TOOL FOR STUDY OF STRUCTURE FOR A RANGE OF MATERIALS INCLUDING MATERIALS CONTAINING LOW-Z ELEMENTS: B, C, AND N. XAS, THE FLUORESCENCE YIELD NEAR EDGE SPECTROSCOPY (FYNES) IN PARTICULAR, REQUIES A… more
Abstract:Linoleic acid, the predominant polyunsaturated fatty acid (PUFA) in the human diet, is a substratecyclooxygenase, lipoxygenase and cytochrome P450 enzymes. The majore metabolitcs of these pathways,13(S)-HODE, 9, 10-epoxyoctadecenoate, and 12, 13-epoxyoctadecenoate are known to exhibit… more
Abstract:The vast majority of studies on prostanoid metabolism have focused on prostaglandin E2, prostacyclithromboxane A2, and the leukotrienes, which are collectively involved in the regulation of a wide rapathophysiological events. Although it has been the subject of less intense investigation, it has… more
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