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AUTOMATED HIGH SPEED SEQUENCING OF DNA

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 16672
Amount: $49,925.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 1991
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
350 Second Ave- Nue
Waltham, MA 02154
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Nappi, Bruce
 Principal Investigator
 (617) 890-3200
Business Contact
 1 R43 HG00528-01
Research Institution
N/A
Abstract

THE OVERALL OBJECTIVE OF THE PROGRAM IS THE DEVELOPMENT OF A PROTOTYPE INSTRUMENT FOR THE AUTOMATED, HIGH SPEED SEQUENCING OF DNA. THE PROJECT WILL COMBINE STATE-OF-THE-ART TECHNIQUES, FOCUSING PARTICULARLY ON THE MULTIPLEXED SEQUENCING AND DIRECT TRANSFER METHODS PIONEERED BY DR. G. CHURCH AND DR. P. RICHTERICH AT HARVARD MEDICAL SCHOOL. IT IS EXPECTED THAT THE COMBINATION OF MULTIPLEXING AND AUTOMATION WILL REDUCE THE COST OF SEQUENCING BY A FACTOR OF 7 TO 10. SPECIFICALLY, THE INTENT IS TO DEVELOP AND COMMERCIALIZE A MEDIUM SIZED MACHINE THAT COMBINES DNA VECTOR TAGGING, AMPLIFICATION, FRAGMENTATION, GEL ELECTROPHORESIS, DIRECT TRANSFER ELECTROPHORESIS, INDIVIDUAL PROBE SIMULATION, FILM RECORDING AND AUTOMATED READING. IN PHASE I THE FOCUS WILL BE ON THE PROBE SIMULATION FUNCTION WHICH IS THE MOST LABOR INTENSIVE ACTIVITY AND THE ONE WITH THE LEAST PRIOR DEVELOPMENT.

* Information listed above is at the time of submission. *

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