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Cloning Hematopoietic Genes by MRNA Differential Display

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1 R43 HL53132-1,
Agency Tracking Number: 25184
Amount: $75,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Solicitation Year: N/A
Award Year: 1994
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
840 Memorial Drive
Cambridge, MA 02139
United States
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Karl Nocka
 (617) 661-3400
Business Contact
Phone: () -
Research Institution

Advances in the use of hematopoietic stem cells for transplantation and gene therapy haveincreased the urgency for a better knowledge of the molecular control of their development andproliferation. An understanding of the molecular processes involved in the earliest stages of stem celldevelopment, currently poorly understood, would provide very useful guide to the selection, expansionand ex-vivo manipulation of stem cells. Our long-term objective is to identify, clone and investigatethese genes. An in vitro assay is now available which allow totipotent murine embryonic stem (ES) cellsto develop into hematopoietic cells. This transition must involve the activatiOn of the earliest geneticprogram for initiating hematopoiesis. By comparison with mRNA species from ES cells that are notdifferentiating into hematopoietic cells, these mRNA may be detected and cloned. A new PCR-basedtechnique has made it possible to obtain "differential display" of mRNA species between two populationof cells. We will combine these two technological advances to identify and clone cDNAs of stem cellrelated genes which could include receptors, transcriptional factors, adhesion molecules and structuralprotein unique and specific to hematopoietic cells. Together they should have a wide range of potentialapplication technologically and commercially.

* Information listed above is at the time of submission. *

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