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COUPLED ENZYME PROCESS FOR TRYPTAMINE SYNTHESIS

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 2R44MH062244-02
Agency Tracking Number: MH062244
Amount: $0.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 2002
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
BIOCATALYTICS, INC. 129 N HILL AVE, STE 103
PASADENA, CA 91105
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 JAMES KILGORE
 (626) 585-9797
 JKILGORE@BIOCATALYTICS.COM
Business Contact
 JAMES ROZZELL
Phone: (626) 585-9797
Email: DAVIDROZZELL@BIOCATALYTICS.COM
Research Institution
N/A
Abstract

Neuroactive tryptamine derivatives can be prepared by a new coupled-enzyme process developed in Phase 1 by combining tryptophan-synthetic and -decarboxylating enzymes. This comprises the first reported single-pot aminoethylation process. The tryptamine products can thereby be conveniently produced from readily-available indole precursors. Expression systems which provide higher enzyme yields will be utilized in order to permit scaleup to preparative reaction systems. The gene encoding Camptothectin accuminata tryptophan decarboxylase 1 (TDC 1) will be redesigned to optimize expression in E. coli. Stepwise binding of tryptophan synthase subunits and mutation of specific sites on the enzyme surface in order to improve enzyme activity following immobilization. Directed evolution will be explored in an effort to broaden the substrate ranges of two enzymes. New substrates will be tested he process will be utilized in the preparation of the migraine drugs rizatriptan, and sumatriptan, as well as melatonin and a series of melatonin receptor active compounds.

* Information listed above is at the time of submission. *

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