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Development of an Advanced Fluidic Sample Bioprocessor

Award Information
Agency: Department of Homeland Security
Branch: N/A
Contract: NBCHC050133
Agency Tracking Number: 0511225
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: H-SB05.1-001
Solicitation Number: N/A
Solicitation Year: 2005
Award Year: 2005
Award Start Date (Proposal Award Date): 2005-06-03
Award End Date (Contract End Date): N/A
Small Business Information
4059 Clipper Court
Fremont, CA 94538
United States
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Stevan Jovanovich
 (925) 413-5952
Business Contact
 Stevan Jovanovich
Phone: (510) 438-9306
Research Institution

This proposal describes a completely automated, NanoBioSentinel sample preparation instrument for biodefense. The NanoBioSentinel will contain two modules: a bead-based Sample Capture and Purification Module (SCPM) front-end and a microchip-based NanoBioProcessor Module. Built partly upon existing devices, the SCPM will use immunocapture and multi-dimensional purifications to process milliliter volumes into microliter volumes. Target organisms and agents are captured, concentrated, and purified on antibody-conjugated beads before lysis followed by bead-based nucleic acid binding and purification in the SCPM. Purified samples on beads are then moved into the NanoBioProcessor Module for fluidic sample processing in a microchip format. The NanoBioProcessor microchips have simple on-chip valves, pumps, and routers and can perform most chemistries. In Phase I, the sample preparation method to be evaluated will be sample preparation for a two-dimensional detection: (1) microscale-Real Time-PCR and (2) microchannel capillary array electrophoresis fragment sizing for confirmation of positives. While we develop the ┬┐backend┬┐ targeting these chemistries, the NanoBioSentinel will be able perform most chemistries and feed most analytical platforms. The technology will be the basis of sample preparation and analysis systems for pathogen detection, diagnostics and biodefense.

* Information listed above is at the time of submission. *

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