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FLUORESCENCE SYSTEM FOR SENSING BIOSPECIFIC INTERACTIONS

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 2R44RR014385-02A2
Agency Tracking Number: RR014385
Amount: $0.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 2002
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
BIOELECTROSPEC, INC. 203 LAVENDER LN
STARKVILLE, MS 39759
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 ALEXANDER ASANOV
 (717) 561-7808
 ASN@BIOELECTROSPEC.COM
Business Contact
 OLGA PETROVA
Phone: (601) 324-1555
Email: OLGA.PETROVA@VERIZON.NET
Research Institution
N/A
Abstract

DESCRIPTION (provided by applicant): An innovative fluorescence
system for sensing biomolecular interactions was developed in Phase I that
is based on total internal reflection fluorescence (TIRF). The main
advantages of this system are integrated microfluidics and a design that
allows rapid replacement of TIRF sensor chip. The feasibility of building a
highly sensitive and versatile TIRF biosensor instrument was successfully
demonstrated in Phase I. In Phase II, we will design and build a complete
turn-key TIRF instrument. The proposed instrument will be equipped with
integrated fluidics, an automated sample handling module and a precision
mechanism for rapid replacement of TIRF sensor chips. A variety of
inexpensive TIRE sensor chips enclosed in cartridges will be available for
convenient immobilization of different biomolecules. In this manner, the
TIRF biosensor instrument will be designed for routine investigation of
biomolecular interactions. During year one of Phase II, we propose to build
five units of the TIRF biosensor instrument. In year two, the TIRF
instrument will be tested in laboratories of our consultants and in our own
lab. At the end of Phase II a fine-tuned industrial prototype wilt be
obtained. The TIRF biosensor instrument will be constructed and tested in
close collaboration with C&L Instruments and our consultants. A TIRF
biosensor instrument is a much-needed tool for studies related to structural
genomics, proteomics and other fields where detailed knowledge of
biomolecular interactions are important. Since the proposed instrument will
transform difficult TIRF experiments into routine measurements, the TIRF
biosensor has the potential to revolutionize studies in many fields,
including cancer research, biomaterials studies, drug discovery, cell
signaling, genomics and proteomics, nanotechnology, immunoassay and vaccine
development.

* Information listed above is at the time of submission. *

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