You are here

Isotopically Labeled Nucleotides for Biomolecular NMR

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R42GM074330-01
Agency Tracking Number: GM074330
Amount: $125,000.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Solicitation Year: N/A
Award Year: 2005
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
Cassia, Llc 12777 Via Felino
Del Mar, CA 92014
United States
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 (858) 784-8740
Business Contact
Phone: (858) 205-3607
Research Institution
LA JOLLA, CA 92037
United States

 Domestic Nonprofit Research Organization

DESCRIPTION (provided by applicant): The goal of the proposed research is to scale up the production and manufacture of high-value ribonucleoside triphosphates containing stable isotope labels. The use for these products is in biomolecular studies of RNA structure using Nuclear Magnetic Resonance (NMR) spectroscopy. The market for these products is structural biology groups in academic and industrial laboratories engaged in structure determination or NMR screening of RNA.
Stable isotope labeling with 13C, 15N, and 2H is a requirement for application of multidimensional NMR studies for structural biology of macromolecules. Existing methods for labeling of RNA and DNA suffer from three main drawbacks. First, the production of isotopically labeled nucleotides is complex and time consuming and difficult to carry out in individual laboratories. Second, it is extremely expensive, since the materials for a single sample can cost many thousands of dollars. Third, the existing methods only permit uniform isotope labeling, while there is considerable advantage to selective isotope labeling.
Recently, we have developed technology that will ameliorate or eliminate these three drawbacks. The technology involves the efficient single-pot synthesis of labeled ribonucleotides from labeled precursors using a series of enzymatic transformations. In order to make these reagents widely available to the structural biology community, we propose to optimize and scale up production of these reagents for commercial distribution.

* Information listed above is at the time of submission. *

US Flag An Official Website of the United States Government