LIGASE CHAIN REACTION AMPLIFICATION FOR DETECTION OF SALMONELLA IN FOODS

Award Information

Agency: Department of Agriculture

Branch: N/A

Contract: N/A

Agency Tracking Number: 14768

Amount: $50,000.00

Phase: Phase I

Program: SBIR

Solicitation Topic Code: N/A

Solicitation Number: N/A

Timeline

Solicitation Year: N/A

Award Year: 1991

Award Start Date (Proposal Award Date): N/A

Award End Date (Contract End Date): N/A

Small Business Information

Biotechnica Diagnostics Inc

61 Moulton St

Cambridge, MA 02138

United States

DUNS: N/A

HUBZone Owned: No

Woman Owned: No

Socially and Economically Disadvantaged: No

Principal Investigator

Name: Keith C Backman
Phone: () -

Business Contact

Phone: () -

Research Institution

N/A

Abstract

THE LIGASE CHAIN REACTION (LCR) ENABLES AS FEW AS 10 DNA TARGETS TO BE AMPLIFIED TO LEVELS DETECTABLE USING MANY CONVENTIONAL DETECTION METHODS. THE AMPLIFICATION TECHNOLOGY CAN BE APPLIED TO THE DETECTION OF SALMONELLA IN FOOD SAMPLES WITH BENEFITS REALIZED IN THE REDUCTION OF PRE-ENRICHMENT AND SELECTIVE ENRICHMENT GROWTH TIMES AND IMPROVEMENT IN ASSAY SENSITIVITY, SURPASSING ALL CURRENT DETECTION ASSAYS. PHASE I OBJECTIVES ARE TO CONSTRUCT A SALMONELLA GENOMIC DNA LIBRARY AND BEGIN IDENTIFICATION OF ONE OR MORE SALMONELLA SEQUENCES OF 30-80 BASES IN LENGTH TOBE USED AS PROBE SETS FOR AMPLIFICATION OF TARGET SALMONELLACELLS IN FOOD SAMPLES. IN PHASE II, DNA CORRESPONDING TO THE SEQUENCES IDENTIFIED WILL BE SYNTHESIZED AND SCREENED FOR ABILITY TO DETECT ALL IMPORTANT SALMONELLA SPECIES AND NOT CROSS-REACT WITH COMMON FOOD-BORNE AND ENTERIC ORGANISMS. THE PROBE SETS THAT FULFILL THESE CRITERIA WILL BE PUT INTO THE LCR ASSAY. IN ADDITION, ISSUES OF SAMPLE PREPARATION, DEFINITION OF LCR CONDITIONS FOR OPTIMAL REACTIVITY AND FOOD TESTING WILL BE UNDERTAKEN.

* Information listed above is at the time of submission. *

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LIGASE CHAIN REACTION AMPLIFICATION FOR DETECTION OF SALMONELLA IN FOODS