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Method for Discovering Novel Transcriptional Regulators

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 2R42GM057712-02A1
Agency Tracking Number: GM057712
Amount: $0.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 2003
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
1 KENDALL SQ, BLDG 600, STE 255 1 KENDALL SQ, BLDG 600, STE 255
Cambridge, MA 02139
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 DUSAN STANOJEVIC
 (617) 549-2238
 DS@CROSSLINKGENETICS.COM
Business Contact
 DUSAN STANOJEVIC
Phone: (617) 549-2238
Email: ds@crosslinkgenetics.com
Research Institution
 WHITEHEAD INSTITUTE FOR BIOMEDICAL RES
 
9 CAMBRIDGE CTR
CAMBRIDGE, MA 02142
United States

 Domestic Nonprofit Research Organization
Abstract

DESCRIPTION (provided by applicant): Regulation of gene expression by transcription factors is one of the most fundamental biological processes. The pharmaceutical manipulation of this process would open doors toward new approaches to treatment of cancer and other major diseases.

We aim to develop a novel, comprehensive technology for manipulation of gene expression based on synthetic analogues of eukaryotic transcription factors. The design and synthesis of these artificial transcription factors (ATFs) has been accomplished in Phase I by replacing functional domains of natural transcription factors with artificial chemical moieties. These ATFs have a remarkably high biological activity combined with novel properties resulting from their unique chemical composition and structure.

In Phase II we propose to develop ATF technology further and apply it to target selected human genes in their native, chromosomal setting in tissue culture cells. This will be achieved by using a combination of chemical, biochemical, genetic and genomic tools. The specific aims of this proposal are:
1) Expanding the repertoire of ATFs with new chemical moieties.
2) Developing a robust and rapid assay for testing of ATFs in human cell culture lines.
3) Demonstration of the ability of ATFs to target selected endogenous human genes in tissue culture.

* Information listed above is at the time of submission. *

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