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Mitochondrial Functional Proteomics

Award Information
Agency: Department of Defense
Branch: Army
Contract: DAAD1903C0117
Agency Tracking Number: A033-0039
Amount: $100,000.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Solicitation Year: N/A
Award Year: 2003
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
11494 Sorrento Valley Rd
San Diego, CA 92121
United States
DUNS: 628050411
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Eoin Fahy
 Director of Informatics
 (858) 509-5611
Business Contact
 Craig Johnson
Title: Chief Financial Officer
Phone: (858) 509-5615
Research Institution
 Shankar Subramaniam Ph.D.
EBU-1 5406, 9500 Gilman Drive
La Jolla, CA 92093
United States

 (858) 822-0986
 Nonprofit College or University

Mitochondria are the organelles that play a central role in energy production in eukaryotic cells. Besides their bioenergetic function, mitochondria regulate cell death, modulate ionic homeostasis, oxidize carbohydrates and fatty acids, and participate inmultiple other catabolic and anabolic pathways. It is estimated that there are 1000-2000 distinct mitochondrial proteins, many of wich have not yet been identified. The invesigators propose to undertake a comprehensive mapping of the mitochondrial proteomeusing mouse heart as a tissue source. Mitochondria will be isolated, purified and separated using a variety of protocols developed and optimized in-house. Detection of low-abundance proteins will be facilitated by first fractionating the mitochondria intotheir various sub-compartments. The protein components of these subfractions will then be subjected to two complimentary mass spectrometry techniques. Data analysis using high-throughput informatics approaches will allow identification of these proteinswith high sensitivity.Furthermore, immunodepletion of high-abundance mitochondrial proteins (such as electron transport chain subunits) using specific antibodies will also be employed to identify mitochondrial proteins present at lower levels in these fractions. Identificationof novel mitochondrial proteins and pathways which are involved in human diseases connected with mitochondrial dysfunction.Elucidation of key mitchondrial intermediates involved in signaling pathways which modulate mitochondrial biogenesis and energy utilization, with a view to developing drugs or food supplements for enhancement of mitchondrial function and physicalperformance.Identification of mitochondrial proteins involved in apoptosis which could be regulated externally to attenuate the aging process.

* Information listed above is at the time of submission. *

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