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Model-driven Strain Design for Pseudomonas Fluorescens

Award Information
Agency: Department of Energy
Branch: N/A
Contract: DE-FG02-03ER83869
Agency Tracking Number: 70441S02-II
Amount: $0.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Solicitation Year: N/A
Award Year: 2003
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
5405 Morehouse Drive Suite 210
San Diego, CA 92121
United States
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Sung Park
 (858) 362-8569
Business Contact
 Christopher Schilling
Phone: (858) 362-8550
Research Institution

70441S02-II To analyze, interpret, and predict the function of biological systems, innovative, integrated approaches are needed to utilize the information generated from genome research. This project will develop novel engineering approaches for studying the systemic capabilities of metabolism in completely sequenced bacterial genomes. In particular, the approach will focus on the development and implementation of a genome-scale metabolic model of Pseudomonas fluorescens. Phase I constructed an in silico metabolic model of P. fluorescens, based in large part on the recently sequenced genome of this organism. The model was refined and validated against existing experimental data on the organism. In Phase II, the in silico model of P. fluorescens, developed in Phase I, will be employed to perform integrated modeling/experimental research to demonstrate the utility of the model for driving metabolic engineering and designing bioprocesses. Experimental validations, of strain design and construction, fermentation evaluation, and gene expression studies, will be conducted. Commercial Applications and Other Benefits as described by awardee: A comprehensive metabolic model of P. fluorescens should be useful in strain engineering of P. fluorescens, which is widely used in the fermentative production of various biologics (e.g. industrial enzymes, and chemicals) as well as in bioremediation treatment strategies.

* Information listed above is at the time of submission. *

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