You are here

Processing Large Volume Samples Using a Silicon Pillar Chip

Award Information
Agency: Department of Defense
Branch: Office for Chemical and Biological Defense
Contract: W911SR-04-P-0054
Agency Tracking Number: C041-106-0102
Amount: $69,783.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: CBD04-106
Solicitation Number: 2004.1
Solicitation Year: 2004
Award Year: 2004
Award Start Date (Proposal Award Date): 2004-05-11
Award End Date (Contract End Date): 2004-11-11
Small Business Information
3918-B Valley Ave.
Pleasanton, CA 94566
United States
DUNS: 037515553
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Phillip Belgrader
 VP and CSO
 (925) 417-7005
Business Contact
 M. Northrup
Title: President and CEO
Phone: (925) 417-7003
Research Institution

PCR and antibody-based devices are currently the primary platforms for bioagent detection. PCR offers high sensitivity and specificity, but has limited effectiveness for toxins (detects residual DNA, if any, in toxin preparations) and requires thermal cycling equipment, extensive sample preparation, and complex reagents. Antibodies, which utilize relatively simple chemistry, are effective for both microbes and toxins, but provide moderate sensitivity and specificity. Therefore, both antibodies and PCR methods will remain important tools for bioagent detection. Much of our attention has been focused on developing automated PCR instrumentation using microfluidics with embedded modular components, such as a minisonicator for cell/spore lysis and a micropillar chip for nucleic acid purification and concentration. For the proposed effort, the micropillar chip, which has an extremely high surface area within a few square millimeters and a liquid volume of only 10 ul, will be derivatized with capture antibodies to rapidly extract microbes and toxins from large volume (>20 ml) samples and concentrate these into small volumes (>0.1 ml). Concentrated agents are then available for both PCR and antibody-based testing.

* Information listed above is at the time of submission. *

US Flag An Official Website of the United States Government