You are here

PRODUCTION OF RECOMBINANT PROTEINS IN MILK

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 7268
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 1987
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
14 Cambridge Center
Cambridge, MA 02142
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 HARRY M MEADE PHD
 (617) 864-8900
Business Contact
Phone: () -
Research Institution
N/A
Abstract

A MODEL SYSTEM FOR PRODUCTION OF RECOMBINANT PROTEINS IN MILK USING MICE WILL BE DEVELOPED IN PHASE I. THIS WILL ESTABLISH THAT IT IS POSSIBLE TO PRODUCE NOVEL PROTEINS IN MILK FROM MAMMARY TISSUES. A PROMOTER SPECIFIC FOR MAMMARY CELLS WILL BE USED TO DIRECT THE EXPRESSION OF A TEST PROTEIN THAT IS EASILY DETECTED. THE DNA CONSTRUCTS CONTAINING THESE SEGMENTS WILL BE INJECTED INTO MOUSE EMBRYOS. THE RESULTING TRANSGENIC MICE WILL BE TESTED FOR THE PRESENCE OF THE NEW RECOMBINANT PROTEINS IN THEIR MILK. THOSE CONSTRUCTS THAT FUNCTION IN THE MOUSE MODEL SYSTEM WILL BE INJECTED INTO BOVINE EMBRYOS IN PHASE II. THE RESULTING TRANSGENIC CATTLE WILL BE TESTED FOR THE PRODUCTION OF RECOMBINANT PROTEINS IN THEIR MILK. THIS PROCEDURE WILL ESTABLISH A METHODOLOGY BY WHICH NOVEL CLONED PROTEINS CAN BE PRODUCED IN COW MILK ON A COMMERCIAL BASIS AT A FRACTION OF THE COST OF CURRENT TISSUE CULTURE TECHNIQUES.

* Information listed above is at the time of submission. *

US Flag An Official Website of the United States Government