THE SPECIFIC AIM OF THIS PHASE I PROJECT IS TO PURIFY TO HOMOGENEITY ACIDIC A-D-MANNOSIDASE FROM HUMAN PLACENTAS AND BOVINE KIDNEYS.

Award Information

Agency: Department of Health and Human Services

Branch: N/A

Contract: N/A

Agency Tracking Number: 4711

Amount: $50,000.00

Phase: Phase I

Program: SBIR

Solicitation Topic Code: N/A

Solicitation Number: N/A

Timeline

Solicitation Year: N/A

Award Year: 1986

Award Start Date (Proposal Award Date): N/A

Award End Date (Contract End Date): N/A

Small Business Information

Genzyme Corp.

75 Kneeland Street

Boston, MA 02111

United States

DUNS: N/A

HUBZone Owned: No

Woman Owned: No

Socially and Economically Disadvantaged: No

Principal Investigator

Name: SHIRISH HIRANI
Title: PRINCIPAL INVESTIGATOR
Phone: (617) 451-1923

Business Contact

Phone: () -

Research Institution

N/A

Abstract

THE SPECIFIC AIM OF THIS PHASE I PROJECT IS TO PURIFY TO HOMOGENEITY ACIDIC A-D-MANNOSIDASE FROM HUMAN PLACENTAS AND BOVINE KIDNEYS. IT IS ANTICIPATED THAT BY EMPLOYING A COMBINATION OF CHROMATOGRAPHIC TECHNIQUES ON AFFINITY, HIGH-PERFORMANCE ANION EXCHANGE, AND/OR HIGH PERFORMANCE GEL FILTRATION MATRICES, HOMOGENEOUS ACIDIC A-D-MANNOSIDASE WILLBE OBTAINED. THIS WILL PERMIT THE GENERATION OF POLYCLONAL AND MONOCLONAL ANTIBODIES DURING PHASE II. FURTHERMORE, THEPURIFIED ENZYME AND ANTISERUM WILL PROVIDE A MEANS FOR CLONING THE GENE FOR ACIDIC A-D-MANNOSIDASE. THE ABSENCE OFTHIS ENZYME IN THE LYSOSOMAL STORAGE DISORDER, MANNOSIDOSIS,WHICH OCCURS IN BOTH MAN AND CATTLE, MAKES IT AN IDEAL CANDIDATE FOR STUDYING THE FEASIBILITY OF GENE REPLACEMENT THERAPY.

* Information listed above is at the time of submission. *

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THE SPECIFIC AIM OF THIS PHASE I PROJECT IS TO PURIFY TO HOMOGENEITY ACIDIC A-D-MANNOSIDASE FROM HUMAN PLACENTAS AND BOVINE KIDNEYS.