You are here

Novel Silica-Based Organic Polymer for Ambient Ionization Mass Spectrometry

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R41GM113337-01
Agency Tracking Number: R41GM113337
Amount: $161,710.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: NIGMS
Solicitation Number: PA14-072
Solicitation Year: 2015
Award Year: 2015
Award Start Date (Proposal Award Date): 2015-01-01
Award End Date (Contract End Date): 2015-12-31
Small Business Information
6500 Technology Center Drive
Indianapolis, IN 46278-6026
United States
DUNS: 626408996
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 (317) 275-5794
Business Contact
Phone: (317) 275-5794
Research Institution
STANFORD, CA 94304-1222
United States

 Nonprofit College or University

DESCRIPTION provided by applicant Most samples of chemical interest are complex mixtures making the usual combination of chromatography with mass spectrometry MS a natural choice However the increased demand for chemical analysis by mass spectrometry in many areas of science makes it imperative to increase efficiency of analysis by minimizing sample workup and overall analysis time which makes direct mixture analysis highly desirable These demands were in many ways solved in with the development of ambient mass spectrometry a branch of mass spectrometry which allows spectra to be recorded on samples in their native state with minimal or no sample preparation Ambient ionization provides the speed needed for in situ experiments by recording mass spectra instantaneously on unmodified samples in their native state and in the ambient environment Sample analytes are loaded onto a substrate material for sampling and supporting the analytes to be studied Nonpolar substrate surfaces currently used in ambient MS offer an advantage over other existing materials in the analysis of a variety of analytes but have limited utility in that they often are impure mechanically weak single use offer poor sample loading have decreased signal intensity and stability and are not easy to modify making them useful for only a particular class of analytes As an alternative to the more widely used sampling materials such as paper and glass we have developed novel organosiloxane OSX polymer materials that have the potential to be used as sampling substrates in ambient ionization methods Our materials can be chemically tuned to increase signal intensity molecule selectivity signal stability and can also be chemically modified by covalent attachment of molecules to serve different analytical purposes within many other possibilities In Aim we will investigate and optimize the synthetic parameters that affect the polymer features such as its porosity polarity hydrophobicity hydrophilicity and its surface features and chemistry In Aim we will use the OSX polymer as a sampling material in spray ionization and desorption electrospray ionization DESI MS to evaluate the performance of the polymer and to identify the optimal operating conditions signal intensity and stability for the polymers investigated and designed in Aim In Aim we will evaluate the feasibility of coupling the polymers to as commercial ambient MS using Prosoliaandapos s commercial robotic sources To assess the utility of these OSX polymers as sampling materials we will analyze drugs in blood serum and study in situ digestion of proteins on polymer surfaces grafted with an enzyme Given the many advantages of OSX polymers they will make excellent commercial products that can widen the application of ambient ionization MS in many different areas including clinical medical diagnostics and proteomics

PUBLIC HEALTH RELEVANCE Ambient mass spectrometry has been increasingly used in many areas of science such as forensics clinical diagnostics and drug analysis as it allows for direct and fast detection of samples However there are limitations in the sampling materials currently used for ambient mass spectrometry We propose to develop and test novel improved sampling materials that can be chemically modified for improving the performance of ambient mass spectrometry analysis and expanding its use

* Information listed above is at the time of submission. *

US Flag An Official Website of the United States Government