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Lab-on-a-chip Flow Cytometer Using COlor-Space-Time (COST) Coding Method

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 2R44GM112442-03
Agency Tracking Number: R44GM112442
Amount: $1,874,535.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: 400
Solicitation Number: PA11-335
Timeline
Solicitation Year: 2016
Award Year: 2014
Award Start Date (Proposal Award Date): 2014-09-01
Award End Date (Contract End Date): 2017-06-30
Small Business Information
7770 REGENTS RD 113390
San Diego, CA 92122-1937
United States
DUNS: 832751098
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 JOSE MORACHIS
 (951) 966-4963
 jose@nanocellect.com
Business Contact
 JOSE MORACHIS
Phone: (951) 966-4963
Email: jose@nanocellect.com
Research Institution
N/A
Abstract

DESCRIPTION provided by applicant Fluorescence activated cell sorting FACS or flow cytometry enables clinicians and researchers to quantitatively characterize the physical cell size shape granularity and biochemical DNA content cell cycle distribution cell surface markers and viability properties of cells With the capability of high throughput sorting to enrih biospecimens and extract rare cell types a state of the art flow cytometer makes it possible to conduct rare event studies such as the identification or isolation of bacterial cells stem cells r tumor cells However current flow cytometers that detect multiple colors and sort cells are expensive $ K complicated hazardous large and bulky For these reasons flow cytometers are often shared amongst labs leading to conflicts in sample handling and scheduling With a growing market expected to reach over $ billion by flow cytometry can address a diverse array of biomedical challenges However there are no high performance flow cytometers with cell sorting capabilities that are affordable for any lab Despite the increasing demand for affordable cell sorters the flow cytometry industry faces fundamental limits in the current technology and its evolutionary path The current system architectures are highly inefficient in accommodating and fully utilizing the increasing number of available fluorescent colors sensitivity ease of use and sorting capabilities We propose an accessible affordable and high performance flow cytometry technology that will allow any scientist to perform cell analysis and sorting in their own laboratory In this Lab to Market Phase II program we propose to dramatically improve our flow cytometric cell sorting platform the WOLF Cell Sorter including extending the dynamic range of the fluorescent detection implementing real time cell sorting verification expanding chip volume manufacturing and validating the system with early adopters such as induced pluripotent stem cell researchers excited by the unique features of our system These technology and business goals will have a direct impact on basic research and clinical applications in human health and disease PUBLIC HEALTH RELEVANCE The advances proposed here will allow NanoCellect to achieve its mission to make cell sorting flow cytometry an everyday lab tool by making our device portable affordable and easy to use Existing cell sorters are prohibitively expensive produce cell damaging shear stress that affect downstream applications create bio hazardous aerosols and require highly trained technicians For emerging markets like stem cell research there is a strong need to develop and make available an affordable user friendly system that is also amenable for highly sensitive cell samples

* Information listed above is at the time of submission. *

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