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A user-friendly scalable microfluidic platform for enhanced neuron-cell culture

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 2R42MH097377-03A1
Agency Tracking Number: R42MH097377
Amount: $1,993,036.00
Phase: Phase II
Program: STTR
Solicitation Topic Code: 101
Solicitation Number: PA15-270
Solicitation Year: 2015
Award Year: 2016
Award Start Date (Proposal Award Date): 2016-04-05
Award End Date (Contract End Date): 2019-09-30
Small Business Information
Temecula, CA 92590-2535
United States
DUNS: 026927928
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 (626) 532-0396
Business Contact
Phone: (626) 532-0396
Research Institution
104 Airport Drive, Suite 2200
CHAPEL HILL, NC 27599-0001
United States

 Nonprofit College or University

DESCRIPTIONprovided by applicantNeuron cell culture is widely used for studies in basic researchdrug discoveryand toxicity testingTraditional neuron cell culture approaches result in random growth of processes which prevent the study of their unique polarized morphologyOur goal is to provide robustuser friendlyand cost effective culture platforms to manipulate and access neurons and their subcellular compartmentsaxonscell body and dendritesData acquired through sales of prototype platforms developed and patented by our team show a large and increasing demandBecause of the unique morphology of neurons and the difficulty in manipulating and studying neurons in vivothis platform has rapidly become an important tool for cell based experimental neuroscienceThese platforms are compatible with high resolution microscopy and allow axons to be manipulated and biochemically analyzedThese prototype platforms are currently replica molded using an optically transparent polymer against master molds that are patterned using photolithographyIn Phase I we developed a new fabrication strategy that will allow us to provide devices that are more uniform and significantly reduce our scrap rate by eliminating cutting or punching of the polymerThis Phase II project will first focu on expanding this fabrication strategy to undertake large scale manufacturing of our platformBased on customer feedback we also identified unmet needs for preassembled devicesdevices optimized for human stem cell derived neuronsand devices amenable to the investigation of synapsesIn this Phase II project we will further focus on these new devices using our feasibility data obtained during the Phase I funding periodThe future commercialization of these devices will scientifically benefit the neuroscience research and testing community by enabling new experimental paradigms

* Information listed above is at the time of submission. *

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