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Single Cell Analysis via Nanoscale Tip Enhanced Laser Ablation Mass Spectrometry

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 2R42GM106454-02
Agency Tracking Number: R42GM106454
Amount: $1,184,346.00
Phase: Phase II
Program: STTR
Solicitation Topic Code: NIGMS
Solicitation Number: PA14-072
Solicitation Year: 2014
Award Year: 2016
Award Start Date (Proposal Award Date): 2016-05-01
Award End Date (Contract End Date): 2018-04-30
Small Business Information
Santa Barbara, CA 93101-3407
United States
DUNS: 556921620
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 (225) 578-3417
Business Contact
Phone: (805) 455-5482
Research Institution
BATON ROUGE, LA 70808-4600
United States

 Nonprofit College or University

DESCRIPTION provided by applicant The goal of the proposed Phase II STTR research is to develop an imaging system capable of detecting and identifying biomolecules in cells and tissue sections with sub cellular spatial resolution The system combines an atomic force microscope AFM with mass spectrometry MS analysis and utilizes a unique tip enhanced laser ablation TELA method developed in the Phase I portion of the research The current technology limitation of imaging mass spectrometry is the fact that it is possible to obtain high resolution images of small molecules and low resolution images of large molecules but it is not possible to both at once The proposed instrument directly addresses this technology limitation The TELA effect developed under Phase I as an off line MS method will be developed under Phase II into an on line instrument that integrates AFM and mass spectrometry imaging for both excellent spatial resolution and biomolecule mass spectrometry The aims of the project are divided into further refinement of TELA and development of the on line TELA MS interface integration of TELA and MS imaging and application to brain imaging Refinement of the tip enhanced laser ablation involves a study of the fundamental interaction between the pulsed ablation laser and the atomic force microscope tip The optimized ablation system will be combined with an ultra low flow electrospray ionization system in which charged particles from the electrospray source interact with the ablation plume to form biomolecule ions Integration of the AFM and MS imaging systems will use four imaging modalities spot by spot sampling area sampling area imaging and depth profiling The result is an AFM capable of both physical and chemical imaging of cells and tissue The AFM TELA MS device will be applied to detection of biomolecules in rat brain tissue Spatial profiling of lipids peptides cocaine and cocaine metabolites will be performed The AFM TELA MS system will be constructed by Anasys based on past product development and manufacturing experience with nanoscale imaging systems such as atomic force microscopy and infrared spectroscopy chemical imaging using tunable pulsed laser sources Mass spectrometry performance tests will be carried out on in the laboratory of Prof Kermit Murray at Louisiana State University who is an expert in the development of laser ablation methods for the detection of biomolecules by mass spectrometry PUBLIC HEALTH RELEVANCE The goal of this project is to develop an imaging instrument capable of identifying biological molecules on the scale of single cells This device addresses the technology limitations of current imaging systems that lack either the ability to resolve singl cells or lack the ability to detect large biomolecules This device is a critical tool for biomedicl research and will assist in the disease research drug development and in clinical diagnosis

* Information listed above is at the time of submission. *

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