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An Innovative Device Integrating Cryopreservation, Storage, and Artificial Insemination of Mouse Sperm

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43OD020304-01A1
Agency Tracking Number: R43OD020304
Amount: $220,199.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: 100
Solicitation Number: PA14-071
Solicitation Year: 2015
Award Year: 2016
Award Start Date (Proposal Award Date): 2016-04-15
Award End Date (Contract End Date): 2018-04-14
Small Business Information
Lexington, KY 40505-3322
United States
DUNS: 178801671
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 (859) 317-9213
Business Contact
Phone: (859) 317-9213
Research Institution

DESCRIPTION provided by applicant ParaTechs Corporation aims to provide researchers with advanced technology and methods to simplify procedures for laboratory animal models While actively supporting efforts to `reduce replace and refineandapos animal use ParaTechs provides safe and efficient alternatives for surgical procedures We have already brought to market a nonsurgical embryo transfer device NSETTM which replaces surgical embryo transfers with a nonsurgical method that takes only seconds and does not require anesthesia We have developed a new protocol for nonsurgical artificial insemination AI of mice which replaces existing protocols for surgical AI and is a technical advancement for existing nonsurgical AI protocols We plan to use this newly developed protocol to revolutionize the process of archiving and global distribution of mouse models through a novel sperm cryopreservation and strain recovery system The use of mouse strains in research is one of the most important tools used to study mammalian gene function While hundreds of thousands of mouse strains have been catalogued and cryopreserved sharing of strains is becoming increasingly difficult as regulatory constraints and the cost of shipping live animals increase We can solve this problem and provide a technically superior alternative with the cryopreservation and insemination Candamp I device This system will integrate storage and shipment of cryopreserved sperm with nonsurgical artificial insemination The device will be suitable for storage of cryopreserved sperm will be shippable will allow viable sperm recovery upon thawing and then will be used directly for nonsurgical artificial insemination This device will eliminate the need to perform in
vitro fertilization IVF upon thawing of cryopreserved sperm greatly reducing the number of mice required for the procedure and replacing a surgical method of artificial insemination both of which promote animal health and welfare Therefore the goals of this project are to design manufacture and test a Candamp I device for mice The goals will be accomplished in two aims First a Candamp I device prototype will be designed and produced Second the feasibility of using the Candamp I device prototype for sperm cryopreservation and mouse strain recovery will be tested This new technological breakthrough will provide an alternative to cryorecovery of mouse strains by in vitro fertilization methods the currently preferred but technically challenging and labor intensiv strain recovery method In doing so the Candamp I device will provide a substantial cost savings to researchers and a convenient method for shipping and strain recovery

PUBLIC HEALTH RELEVANCE The ability to genetically modify the laboratory mouse is now producing an ever rising number of increasingly valuable and unique strains to investigate human disease While maintaining these mouse strains has become a significant and recurring effort and budgetary commitment cryopreservation of strains in sperm repositories has proven successful as a means to permanently and economically preserve them ParaTechs proposes to design manufacture and test a cryopreservation and insemination Candamp I device for the purpose of integrating cryopreservation storage shipping and strain recovery of mice through a newly developed nonsurgical artificial insemination method

* Information listed above is at the time of submission. *

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