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Breakthrough sub cellular D chemical composition research platform for live cells based on the new invention of nanoscale confocal photothermal IR micro spectroscopy n CPIR

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R41CA224844-01
Agency Tracking Number: R41CA224844
Amount: $225,000.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: 100
Solicitation Number: PA17-148
Timeline
Solicitation Year: 2017
Award Year: 2017
Award Start Date (Proposal Award Date): 2017-09-30
Award End Date (Contract End Date): 2018-03-31
Small Business Information
325 CHAPALA ST.
Santa Barbara, CA 93101-3407
United States
DUNS: 556921620
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 JIXIN CHENG
 (617) 353-1276
 jxcheng@bu.edu
Business Contact
 ROSHAN SHETTY
Phone: (805) 455-5482
Email: roshan@anasysinstruments.com
Research Institution
 BOSTON UNIVERSITY MEDICAL CAMPUS
 
85 EAST NEWTON STREET, M-921
BOSTON, MA 02118-2841
United States

 Nonprofit College or University
Abstract

Abstract
This proposal will lead to a Breakthrough sub cellular D chemical composition research platform for live
cells based on the new invention of sub micron confocal photothermal IR micro spectroscopy CPIR
Label free sub micron chemical imaging and spectroscopy has long been sought for visualization of
biomolecules and materials in complex living systems Many diseases first manifest themselves at the
cellular or subcellular level IR micro spectroscopy is a powerful technique but its use in life sciences in
general and cellular analysis in particular has been limited due to the following key limitations
a Spatial resolution limited by diffraction to m
b Inability for in vivo imaging due to strong IR absorption by water
This proposal eliminates both of the above limitations and is based on a recently published patent
pending breakthroughby the PI Prof Ji Xin Cheng of Boston University and is licensed exclusively by
Anasys Instruments for commercialization Prof Cheng is one of the worldandapos s leading vibrational
spectroscopy researchers and is the co inventor of the CARS Coherent Anti Stokes Raman Scattering
microscope which is a major innovation in the field of Raman Spectroscopy Anasys pioneered the field
of AFM Atomic force microscope based photothermal nanoscale IR Spectroscopy where an AFM
probe detects the photothermal signal induced by IR absorption
The goal of this proposal is to create a high speed confocal optical microscopy platform capable of
nm IR Spectroscopy in fluid This platform can be used in sub cellular chemical composition research of
live cells which has many applications in the life sciences
The proposed CPIR platform is highly innovative because it would enable real time imaging of lipid
metabolites in single live tumor cells based on fingerprint IR bands Compared to the widely studied
genetic aspect of cancer and the well known Warburg effect appreciation of the role of lipids in cancer
development is still emerging Aberrant expressions of lipogenic genes have been found in brain
mammary prostate and many other cancer Even with these discoveries lipid metabolism has not been
used as a prognostic factor for cancer aggressiveness due to lack of differential detection and
quantitation technology Our proposal aims to fill this gap by quantifying the amount and composition of
lipid droplets an important aspect of lipogenesis in tumor cells Though we focus on lipid metabolites
here our spectroscopic imaging platform is generally applicable to monitor the intracellular dynamics of
other metabolites anti cancer drugs and nutrition molecules such as fatty acids and amino acids thus
having a far reaching impact on cancer research Project Narrative

The goal of this proposal is to create a sub cellular D chemical composition research platform for live
cells based on the new invention of sub micron confocal photothermal IR micro spectroscopy It will
a Provide nm spatial resolution for IR imaging and Spectroscopy at high speeds b Work in fluid

* Information listed above is at the time of submission. *

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