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iFACS: Imaging Florescence Activated Cell Sorter to sort cells based on images

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 2R44DA042636-02
Agency Tracking Number: R44DA042636
Amount: $1,499,912.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: NIDA
Solicitation Number: PA16-302
Timeline
Solicitation Year: 2016
Award Year: 2017
Award Start Date (Proposal Award Date): 2017-09-30
Award End Date (Contract End Date): 2019-08-31
Small Business Information
7770 REGENTS RD 113390
San Diego, CA 92122-1937
United States
DUNS: 832751098
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 SUNGHWAN CHO
 (858) 414-4147
 scho@nanosort.net
Business Contact
 NATE HEINTZMAN
Phone: (858) 699-5230
Email: nate@nanocellect.com
Research Institution
N/A
Abstract

iFACSImaging Florescence Activated Cell Sorter to sort cells based on images
NanoCellect BiomedicalIncRESEARCH and RELATED Other Project InformationPROJECT SUMMARY
Advances in reagentse gCRiSPRand analytical toolse gflow cytometershave improved the ability to
alter and characterize cellular phenotypesUltimatelymany key applications in biomedicine require efficient
and accurate isolation of cell populations according to features contained in high content imagesUnfortunatelymicroscopic laser microdissection systems have a throughput that is too slow to be practical in
many applicationswhile the existing flow cytometers that can sort cellsfluorescence activated cell sorters or
FACSprovide only sizeinternal complexityand fluorescence intensity information and lack the rich data of
imagingAnother critical limitation is that the existing flow cytometers that can imagecannot sort cellsNanoCellect has made a highly affordable FACS to increase access to this important high throughput tool for
cell analysisHere we propose to enhance our existing low cost FACS with the ability to image cells and sort
them based on image featuresThis will allow users to pursue new strategies in drug screening and
mechanism of action researchas well as work with suspension cell linessuch as those that dominate the
recent advances in immuno oncologyIn Phase I researchwe have demonstrated the world s first imaging flow cytometer with cell sorting
capabilitiesiFACSin a unique design of space time coding with an optical spatial filterThe approach adds
negligible cost to the system for the desirable features of cell imaging and sortingTo fully realize the enormous potential of the design and to meet the demands for most applicationsin Phase
II we will develop high throughput image based cell sorting with innovative image guided gating schemes
supported by machine learning and interactive user machine interfaceEssentiallyimage based flow
cytometry gating uses similar cell isolation criteria as the techniques of laser capture microdissection
or cell aspiration to isolate cells of interestwithX throughput improvements tocells per
secondWe envision such unique capabilities will become commondefault features for tomorrow s users as the tool
becomes as intuitive and ubiquitous as fluorescent microscopyThe proposed iFACS will be transformative
and benefit numerous biomedical applicationssuch as isolation of cells based on organelle translocationcell
cycle analysesdetection and counting of phagocytosed particlesand protein co localizationto name just a
few

* Information listed above is at the time of submission. *

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