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Novel isotope sensor for spatially-resolved analysis of nutrient exchange in the rhizosphere Topic: 22.a. Technologies for Characterizing the Rhizosphere: Plant-Microbe-Mineral Interactions

Award Information
Agency: Department of Energy
Branch: N/A
Contract: DE-SC0018488
Agency Tracking Number: 0000234791
Amount: $225,004.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: 22a
Solicitation Number: DE-FOA-0001770
Timeline
Solicitation Year: 2018
Award Year: 2018
Award Start Date (Proposal Award Date): 2018-04-09
Award End Date (Contract End Date): 2019-04-08
Small Business Information
19805 Hamilton Ave.
Torrance, CA 90502-1341
United States
DUNS: 625511050
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Jason Kriesel
 (310) 756-0520
 jason@oksi.com
Business Contact
 Jason Kriesel
Phone: (310) 756-0520
Email: jason@oksi.com
Research Institution
 Pacific Northwest National Laboratory
 James Moran
 
902 Batelle Blvd.
Richland, WA 99354-1793
United States

 (509) 371-6798
 Federally Funded R&D Center (FFRDC)
Abstract

Ensuring adequate agricultural productivity in the face of an increasing human population is a major concern with implications for both production of bioenergy products (Jones and Hinsinger, 2008) and global food security. Increasing agricultural productivity requires a stronger understanding of soil biogeochemical processes that impact plant nutrient availability. We propose development of an innovative isotope sensor that will enable high spatial-resolution analysis (down to the microbe level) to target nutrient exchange in the rhizosphere. The concept uses laser ablation to sample specific locations in the roots and rhizosphere at the micron (┬Ám) scale. The resulting, sample- derived ablation particulates are converted to a gas and then analyzed for isotopic content. Specifically, we propose using a novel, ultra-low volume isotope analyzer based on laser absorption spectroscopy, which will initially be used to analyze stable carbon isotope ratios (13C/12C) at high spatial resolution. The resulting tool will enable studies designed to track uptake of isotope labeled 13CO2 from fixation into the plant and eventually through the roots and into the soil as root exudates. The concept builds on successful work in which laser ablation has been coupled to isotope ratio mass spectrometers (IRMS). The innovative aspect is that the IRMS will be replaced by a novel, more sensitive laser absorption spectrometer, which can analyze orders of magnitude smaller sample sizes, enabling smaller ablation spot sizes and improved spatial resolution. Furthermore, as compared to IRMS (as well as other isotope analyzers), our laser absorption spectrometer is more compact, lower power, and more amenable to potential field deployment.

* Information listed above is at the time of submission. *

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