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Development of a Rapid Monitoring System for Detection of Pathogens in Marine Aquaculture Operations

Award Information
Agency: Department of Commerce
Branch: National Oceanic and Atmospheric Administration
Contract: 1305M218CNRMW0062
Agency Tracking Number: 18-1-114
Amount: $120,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: 8.3.6
Solicitation Number: NOAA-2018-1
Solicitation Year: 2018
Award Year: 2018
Award Start Date (Proposal Award Date): 2018-07-17
Award End Date (Contract End Date): 2019-01-16
Small Business Information
132 East Main Street #68
Drexel, MO 64742-0068
United States
DUNS: 830979048
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Michael Hornback
 Senior Scientist
 (816) 619-3375
Business Contact
 Andrew Page
Title: President and CTO
Phone: (816) 619-3375
Research Institution

TECHNICAL ABSTRACT: InnovaPrep proposes to develop a rapid sample-to-answer monitoring system for detecting pathogens in marine aquaculture waters. An assessment of industry needs will include a kickoff meeting with NOAA and a visit to Toby Island Bay Oyster Farm. Following the assessment and report, a TRL-4 breadboard prototype system will be developed that will include the combination of sample concentration, cell lysis, and DNA purification. Finally, the system will be demonstrated at two concentrations using the BSL-1 species,Vibrio harveyi, as a surrogate pathogen. The bacteria will be spiked into simulated seawater at 0.1 CFU/mL (low level) and 10 CFU/mL (high level). The final prepared samples will be analyzed by real-time quantitative Polymerase Chain Reaction. An InnovaPrep funded, preliminary marketing study will also be performed during Phase I.SUMMARY OF ANTICIPATED RESULTS: Anticipated results of the Phase I effort and the parallel marketing study, include a market size estimate for a commercial version of a mature system, and data confirming that such a system can achieve detection levels that are significant to the aquaculture industry. We anticipate confirming that the prototype system will achieve >700X concentration of analysis-ready DNA from 1L of seawater and detection of the surrogate at 285 CFU/mL.

* Information listed above is at the time of submission. *

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