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Protein ProFiler Microfluidic Electrophoresis System for Proteomics

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R41GM130206-01
Agency Tracking Number: R41GM130206
Amount: $477,539.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: 400
Solicitation Number: PA17-303
Timeline
Solicitation Year: 2017
Award Year: 2018
Award Start Date (Proposal Award Date): 2018-09-20
Award End Date (Contract End Date): 2019-09-19
Small Business Information
100 WILSHIRE BLVD, STE 700
Santa Monica, CA 90401-3602
United States
DUNS: 080585207
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 JOHN WIKTOROWICZ
 (408) 960-7457
 jwiktor@innovaregi.com
Business Contact
 JOHN WIKTOROWICZ
Phone: (409) 772-2764
Email: jowiktor@utmb.edu
Research Institution
 UNIVERSITY OF TEXAS MED BR GALVESTON
 
301 UNIVERSITY BLVD
GALVESTON, TX 77555-5302
United States

 Nonprofit College or University
Abstract

AbstractThe current estimate of the number of human genes is aboutin contrast to the
number of presumed proteinswhich likely exceeds one millionThis increased complexity is the
result of post transcriptional and post translational modificationsPTMsoccurring at multiple sites
and in a quantitative variable mannerThe overwhelming result is a combinatorially expanding
complexity of proteoforms that is a challenge to proteomic studies in general and the study of
biological mechanisms in particularAmong the new tools utilized in this efforttop down mass
spectrometryTD MSanalyzes intact proteins with capable mass spectrometersand in doing sois ideally suited towards preserving the complexity of multiply modified proteinsHoweverbecause
the larger intact proteins acquire multiple charged states in the MSpeak capacity is significantly
limitedAs a consequencedeep mining of the proteome by TD MS is extraordinarily reliant on
highly resolving protein separations and quantitative recoveryMost separation systems historically
utilized for conventional proteomic mass spectrometry rely on separation systems ideally suited for
peptidenot intact proteinsHence there is high demand for efficientrapidand quantitative
separations systemsspecially designed for intact proteins and TD MSThe lack of such
separations technology is a major roadblock to TD MS progress and its wider acceptanceWe have developed a separations device that utilizes a specially designed and patented
microfluidic glass plate with proprietary coatings that achieves high resolution separations of intact
proteins based on truly orthogonal protein propertiessize and chargeWhile similar in principle to
conventionalD gel electrophoresisour microfluidic Protein ProFileravoids rigid gelsMSincompatible denaturing detergentse gSDSand accomplishes separations by sizefollowed
by isoelectric focusing to achieve the highest sensitivityCollection of proteins is accomplished by
selective or non selective meanspermitting tailoring the population of collected proteins to the
goals of the experiment and MS capabilitiesPreviously we have demonstrated high performance
metrics of the system one dimension at a timeHoweverthis project is focused on completion of
the quantitative protein recovery subsystemencompassed in one specific aimSuccessful
achievement of the milestone underlying the specific aim and ultimate commercialization
will not only demonstrate intact protein separations with quantitative recovery in an
automatedMS compatibleliquid based microfluidic platformbut will play an impactfulenabling role for the TD MS proteomics communityeffectively providing the necessary
tools to decipher the PTM challenges of modern biology Project Narrative
This project will complete development of the final subsystemprotein collection
subsystemto allow integration into the larger much needed separations
technology for top down proteomicscalled the Protein ProFilerThe ProFiler is
a microfluidicself contained two dimensional electrophoresis deviceseparating
proteins by size using an flowable liquid polymer and isoelectric point using
covalently bound pH buffersThe subsystem will recover separated intact
proteins in a format compatible with a top down proteomics approachthereby
enhancing the acceptance and enabling ultimate realization of the promise of
proteomics and its application to precision medicine

* Information listed above is at the time of submission. *

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