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Fluorescent nanofoldamers for multiplex flow cytometry

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R41GM131501-01
Agency Tracking Number: R41GM131501
Amount: $300,000.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: 400
Solicitation Number: PA18-575
Timeline
Solicitation Year: 2018
Award Year: 2018
Award Start Date (Proposal Award Date): 2018-09-17
Award End Date (Contract End Date): 2019-09-16
Small Business Information
2 DAVIS DR STE 204
Research Triangle Park, NC 27709-0003
United States
DUNS: 078769570
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 JAMES PITNER
 (919) 354-1053
 bruce@nirvanasciences.com
Business Contact
 RUSSELL THOMAS
Phone: (919) 354-1053
Email: russell@nirvanasciences.com
Research Institution
 NORTH CAROLINA STATE UNIVERSITY RALEIGH
 
2701 Sullivan Drive, Suite 240
RALEIGH, NC 27695-0001
United States

 Nonprofit College or University
Abstract

PROJECT SUMMARY ABSTRACT
Polychromatic flow cytometryFCis one of the most powerful analytical techniques used in immunologybasic
researchand clinical medicineIn basic researchFC is a primary tool for understanding disease development
at the cellular and subcellular levelsIn the rapidly developing field of immunotherapyFC is an indispensable
tool for monitoring the effectiveness of new therapies and identifying key cellular subpopulations using multiple
biomarkers within a panelIt plays a critical role in several growing clinical applicationsdevelopment of
vaccines for infectious diseasesincluding emerging diseases such as Ebola and Zikacharacterization of
immunological cells and responses related to transplantation and treatment of graft versus host diseaseand
development of vaccines and immune therapies to prevent and treat HIV infectionsIncreasing the number of
spectrally distinct fluorophores for analysis of a single sample will give researchers greater flexibility and give
clinicians a higher level of accuracy in the diagnosis and management of conditions where the size of the
sample use for diagnosis is limited and for specimens with low cell numberssuch as those derived from fine
needle aspirationlaparoscopycore biopsyand cerebrospinal fluidDue to these small sample sizesthere
are often insufficient cell numbers for multiple panelsImprovements in optics and lasers have driven the
recent introduction of new fluorophores for FCHowevermost of these new fluorophores have broad and often
overlapping emission profilesmuch like traditional fluorophoresOverlapping spectra can be resolved by the
combined use of bandpass filters and mathematical compensationoverlap subtractionhowever such
compensation increases experimental errorreduces sensitivityand limits multiplexingThuseven with a
prototypecolor instrumentleading investigators have only been able to implement aparameter paneladvances in reader technology have outstripped available capabilities in fluorophoresNIRvana Sciences is currently developing two families of tetrapyrrole fluorophores licensed from NC State
UniversityThese fluorophores offer properties that make them excellent candidates for use in polychromatic
FC including ultra violet violet excitationlong stokes shifts into the red and near infrared spectrums along with
very narrow emissionsNIRvana Sciences and the Lindsey lab are developing a polymer platform that retains
the intrinsic brightness of its dyes and has strong utility to the flow cytometry reagent market PROJECT NARRATIVEPUBLIC HEALTH RELEVANCE
Flow cytometry is used routinely for both basic research and clinical diagnostic applicationsMany types of
infectious diseases and autoimmunity are routinely diagnosed using flow cytometryand it is widely used in the
development of vaccines and immunotherapies for cancerThis STTR project will advance the development of
a new technology for creating bright and sharp dyes in the red to far red spectral regionwhich will greatly
expand the suite ofcolorsavailable for multiplexed flow cytometry and allow for higher sensitivitysmaller
sample sizeand higher throughput testing

* Information listed above is at the time of submission. *

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