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Phase II Commercialization of HABStats: A Harmful Algal Bloom Species, Toxin and Total Phytoplankton Identification System

Award Information
Agency: Department of Commerce
Branch: National Oceanic and Atmospheric Administration
Contract: 1305M219CNRMW0021
Agency Tracking Number: 18-2-071
Amount: $400,000.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: 8.2.1
Solicitation Number: NOAA-2018-2
Solicitation Year: 2019
Award Year: 2019
Award Start Date (Proposal Award Date): 2019-06-27
Award End Date (Contract End Date): 2022-06-23
Small Business Information
10 Edgerton Drive
North Falmouth, MA 02556
United States
DUNS: 080082679
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: Unavailable
Principal Investigator
 Scott Gallager
 (508) 472-5520
Business Contact
 Fred  Richards
Title: CEO
Phone: (508) 472-5520
Research Institution

In Phase I, we developed a prototype instrument for counting, sizing and identifying both non-toxic and toxic Harmful Algal Bloom (HAB) species. We did this by integrating three innovative technologies: High-throughput color, Light Field Imaging Flow Cytometry to acquire morphological information for each cell, Raman spectroscopy to acquire species-specific molecular information on pigments and triglycerides, and Convolutional Deep Neural Networks providing Deep Learning data integration and an artificial taxonomist. We showed that at least 10 to 100 cells per second could be processed in real-time. We built an image and Raman library of more than 22 marine and 30 freshwater HAB species. Several HAB toxins were characterized and quantified by Raman spectroscopy. Phase II Objectives: continue building
Raman libraries focusing on HAB toxins; examine the specificity of Raman compared to HPLC and ELISA analysis of HAB toxins; complete integration of 3-D imaging, Raman spectroscopy, and Deep Learning classification of cells and toxins into a Gen2 prototype that may be provided to beta-testers; work with Lockheed Martin to develop a Design for Manufacturing Plan for the HABStats instrument; construct four Gen2 HABStats and provide them to beta-testers involved in management and research of both marine and freshwater HABs.

* Information listed above is at the time of submission. *

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