Developing a Scalable Manufacturing Process for an Oral Bacteriophage Product that Prevents Cholera

PROJECT SUMMARY
Cholera is an acute and severe disease caused by the bacterium Vibrio cholerae that is spread primarily
through contaminated water sources due to a lack of adequate sanitation infrastructure. The World Health
Organization estimates that there are at least 3 million cases globally per year, 40 percent of which are spread
through household transmission. Current prevention methods require significant investment of resources and
time for efficacy, but household contacts of cholera patients often present with cholera symptoms two to three
days after the initial patient becomes sick. In addition, the preventive use of antibiotics is not recommended
due to widespread resistance and the known negative consequences of dysbiosis. There is a pressing need to
develop a targeted clinical intervention to prevent the community spread of cholera using a rapid prophylactic
treatment. The goal of the proposed studies is to demonstrate feasibility of developing our phage-based clinical
intervention, ProphaLytic-VC, to prevent the community spread of cholera. ProphaLytic-VC is an orally
administered cocktail of three different phages known as ICP1, ICP2, and ICP3. To date, these are the only
three virulent phages found in association with epidemic strains of cholera. Detailed genomic and phenotypic
characterization each of the phages has been conducted by the academic partner, resulting in genome
sequences, annotation, and identification of the host receptors. The combination of the three has been
evaluated to demonstrate potency in reducing colonization of V. cholerae and prevention of cholera symptoms
in two animal models of V. cholerae colonization and pathogenesis. Here, we propose studies to optimize a
robust and scalable manufacturing process for ProphaLytic-VC, as there is currently little manufacturing
experience in the USA due to the novel nature of the product. We will construct two phage production strains,
develop a scalable phage production and purification scheme, and evaluate any potential local of systemic
toxicity. At the successful conclusion of Phase I, we will have a resultant Master Phage Stock (MPS) of
ProphaLytic-VC from an optimized large-scale production scheme that is potent against V. cholerae and safe
with repeated oral administration. STTR Phase II funding will be used to fund further activities to generate a
working phage seed stock from the MPS to proceed with a Phase I clinical trial.PROJECT NARRATIVE
This proposal aims to demonstrate the feasibility of developing a phage-based clinical intervention to prevent
the community spread of cholera. The academic partner has conducted preclinical studies on the innovative
product (ProphaLytic-VC) to show efficacy. Together with the small business partner, we are aiming to
develop a robust, scalable manufacturing process for ProphaLytic-VC that is stable and safe for use in a Phase
I clinical trial, as there is currently lack of production experience in the USA due to the novel nature of the
product. The success of this study will result in an optimized large-scale phage production and purification
scheme for ProphaLytic-VC, which can then be safely administered to household contacts of cholera patients
to protect those most at-risk for infection and make a significant impact on the global control of cholera.