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No burden / low burden biological air sampler

Description:

TECHNOLOGY AREA(S): Chem Bio Defense

OBJECTIVE: To develop a passive biological air sampler that integrates into current Personal Protective Equipment (PPE) ensembles.It should be easily deployed and tested in all / many current fieldable biological detection platforms.

DESCRIPTION: Novel technology is needed to overcome the shortcomings associated with this lack of portability and burdensome logistical train associated with biological air samplers.Innovative sampling solutions are sought that integrate with limited burden into or onto personal protective equipment (PPE) while eliminating the need for an externally powered air sampler, maintenance, and the overall logistic burden associated with processing the sample matrix from deployment to use in fielded biological detectors.Potential solutions may include novel media (e.g., gels or adsorbent fabrics), passive concentrating technology, or integrated into / onto current fielded respiratory systems.The goal is to develop a lower maintenance, lower profile, and lighter weight biological air sample matrix add on / passive flow device for end user exposure surveillance.Current technology turnaround time to gather actionable knowledge of the presence of a biological in the air is far too long. This technology developed would reduce that time and reduce the burden of actively gathering an air sample.Potential operational uses may include prolonged entry into immediately dangerous to life and health (IDLH) atmospheres or unknown CBRN environments.

PHASE I: Design and develop passive, IPE integrated biological sampling technology / matrices that are effective in passively capturing particles present in the air and offer significant improvements in the logistical burden associated with the current air sampling technologies.Demonstrate feasibility in application, integration in COTS prep kits and archive capable and ability to have biological material capture in/on the material.Identify key performance efficiency parameters and test criteria relevant to use and follow on testing with one biological test platform.Establish test set up and procedures to assess and validate proposed approach.Fabricate physical model and conduct bench top testing to characterize the performance of the proposed solution(s) to include but not limited to the following, where applicable to the proposed technology:adsorption capacity, efficiency of capture rate, and use in diagnostic platform detailing limitations (if any), logistic requirements, inhibition by-products, and efficiency under relevant temperature/humidity conditions.

PHASE II: Refine and optimize Phase I model to capture protein/toxin and bacteria capacity and performance.Perform follow-on bench top evaluations to verify improvements made to the basic enabling technology.Modify test setup and improve test procedures as required.Develop functional prototype by incorporating enabling technology into a suitable test bed (e.g., M50 mask or UIPE ensemble). This technology should be non-invasive to any PPE and capable of being donned / doffed while fully protected.Characterize test bed performance under an operational relevant range of external environmental (temperature/humidity) conditions.Based on results obtained, implement necessary refinements to the scalable test bed model.In preparation for Phase III, transfer functional prototype to CCDC CBC for independent benchtop verification and validation. Training of Government personnel will be provided by the performer in the proper use of the prototype.Performer will offer test support including addressing technical issues.

PHASE III: Fully integrate solution into a full-scale, fully-functional prototype.Demonstrate ability of the technology to be incorporated into an end user donned ensemble through modification of an existing systems.Expand applications to other commercial detectors.Transfer fully-functional prototype to CCDC CBC for independent verification and validation in aerosol chamber.Training of Government personnel will be provided by the performer in the proper use of the prototype.Performer will offer test support including addressing technical issues.

KEYWORDS: Biological Sampler, Passive, Integrated, Low burden

References:

Biomarkers: Potential Uses and Limitations https://www.ncbi.nlm.nih.gov/pmc/articles/PMC534923/pdf/neurorx001000182.pdf; Exposure assessment โ€“ an introduction. http://sphweb.bumc.bu.edu/otlt/mph-modules/ExposureAssessment/exposureassessment9.html; Trends in Passive Sampling. TrAC Trends in Analytical Chemistry 21(4):276โ€“291 ยท April 2004; Passive Sampling Techniques in Environmental Monitoring, Volume 48 1st Edition May 2007 ISBN: 9780444522252; Appl. Environ. Microbiol. doi:10.1128AEM.01589-18 / September 2018

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