Detection of “active” biological toxins by exploiting native binding specificities

A chemical threat agent mode of action is often initiated through a selective interaction with a biological molecule or structure. The binding event mimics normal cellular processes, but then overruns natural control mechanisms leading to toxic and lethal effects. The native binding specificity is used to design sensor recognition elements that outperform conventional antibody-based biosensors. Our custom developed interfaces allow for high loading of the recognition molecules on the transducer, increasing its sensitivity and dynamic range. Additionally, extended stability of the standard recognition elements and custom high-performance transducer provides sufficient storage and operation lifetimes for field application. Another advantage gained by using the native interaction mechanism for detection combined with the transducer design reduces “false-positives” and increases reliability. Only the active agent binds its native target molecule, unlike antibody-based detection where similar cross-reacting biomolecules or denatured agents initiate a signal. The concept limits interference and efforts that may attempt to mask or mimic chemical threats. The Sequential Phase II project focuses on optimization and performance improvement, and on aggressive increase of the technical readiness level (TRL). The Sequential Phase II project aims to develop a fully functional industrial prototype of the detector.