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Production of a stable recombinant human butyrylcholinesterase

Award Information
Agency: Department of Defense
Branch: Office for Chemical and Biological Defense
Contract: W911QY-20-P-0035
Agency Tracking Number: C192-007-0051
Amount: $167,481.06
Phase: Phase I
Program: SBIR
Solicitation Topic Code: CBD192-007
Solicitation Number: 19.2
Solicitation Year: 2019
Award Year: 2020
Award Start Date (Proposal Award Date): 2020-04-03
Award End Date (Contract End Date): 2020-09-30
Small Business Information
737 Concord Ave
Cambridge, MA 02138
United States
DUNS: 080316838
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Piercen Oliver
 Director of Research and Development
 (617) 621-8500
Business Contact
 Peter Howe
Phone: (617) 621-8500
Research Institution

Human butyrylcholinesterase (BChE) is an effective bioscavenger of organophosphates and has potential for acting as a pre-exposure prophylactic or post-exposure treatment for organophosphate (OP) nerve agents, such as sarin. Ideally, a single dose of BChE could be administered and protect the warfighter for days or even weeks while in combat. To be effective in a military setting, especially as a prophylactic, BChE must have a very long serum half-life and must be non-immunogenic. It is now well-established that the plasma half-life of recombinant BChE is strongly affected by the extent of its oligomerization, glycosylation, and sialylation. Clear Scientific (CS) will partner with General Biologics, Inc. (GB), to design, express, and purify recombinant human BChE that meets or exceeds the blood serum half-life of wild-type human BChE. The team will use a multi-faceted approach to confer the requisite properties of BChE (full sialylation and tetrameric form). By the end of Phase I, CS and GB will have determined the best mutant BChE candidates for sialylation optimization, pharmacokinetic (PK) studies in small animals, lead optimization, and scale-up to grams of product in Phase II.

* Information listed above is at the time of submission. *

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