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Commercialization of methods and reagents for high-throughput single cell analysis

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 2R44GM137648-02
Agency Tracking Number: R44GM137648
Amount: $1,146,224.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: 400
Solicitation Number: PA22-176
Timeline
Solicitation Year: 2022
Award Year: 2023
Award Start Date (Proposal Award Date): 2023-09-01
Award End Date (Contract End Date): 2025-07-31
Small Business Information
15 PALMER ST
Watertown, MA 02472-2757
United States
DUNS: 081277504
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: Yes
Principal Investigator
 KRISTINA FONTANEZ
 (857) 600-2962
 kfontanez@fluentbio.com
Business Contact
 SEPEHR KIANI
Phone: (781) 552-6171
Email: skiani@fluentbio.com
Research Institution
N/A
Abstract

PROJECT SUMMARY / ABSTRACT
Single-cell RNA sequencing (scRNAseq) provides unprecedented insight into the gene regulatory pathways of
living cells in complex tissues. Fluent BioSciences has developed Pretemplated Instant Partitioning for
single-cell RNA sequencing (PIPseq) that eliminates the cost and complexity of bespoke microfluidics and
instrumentation and features a simple, flexible workflow that can be implemented in any laboratory setting.
With Phase I SBIR support (1 R43 GM137648-01A1) we have demonstrated that PIPseq is intrinsically
scalable and is capable of capturing from hundreds to millions of cells in a single reaction. The advent of new,
high capacity, and low cost sequencing modalities such as that developed by Ultima Genomics makes high
cell-input single cell transcriptomics not only feasible but practical, enabling previously inaccessible
experimental modalities. In this proposal, we will expand the capabilities of PIPseq single cell analysis to
provide simple, scalable, and cost-effective solutions for high cell input applications including deep tissue
profiling, multiplexed drug testing, and pooled CRISPR screening.

* Information listed above is at the time of submission. *

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