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HIGH THROUGHPUT GENOTYPING TECHNOLOGY

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R41HG002560-01
Agency Tracking Number: HG002560
Amount: $98,312.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 2002
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
PARALLELE BIOSCIENCE 384 OYSTER POINT BLVD, STE 8
SOUTH SAN FRANCISCO, CA 94080
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 THOMAS WILLIS
 (650) 228-0355
 TOM@P-GENE.COM
Business Contact
 MELINDA THOMAS
Phone: (650) 228-0356
Email: MELINDA@P-GENE.COM
Research Institution
N/A
Abstract

DESCRIPTION (provided by applicant): There is a need for inexpensive high
throughput platforms for analyzing large number of single nucleotide
polymorphisms. In this proposal, we describe the development of a technique,
which will enable more than 10,000 SNPs to be analyzed in a single tube and its
transfer to a commercial entity (ParAllele Genomcis Inc.). The technology
exploits the specificity of padlock probes, and employs the fidelity of
polymerase and ligase to accurately identify the nucleotide polymorphism. A
molecular tag is designed in the backbone of the probe to allow parallel
detection of many polymorphisms. In this way, the information of the
polymorphism can be reformatted into the tag sequence, which is then amplified
using general PCR primer. The amplification product is then screened on a
complementary tag chip. This technology is expected to have applications in
population genetics and large-scale disease association studies and unlike
other available technologies uses very low amount of DNA sample per SNP
screened.

* Information listed above is at the time of submission. *

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