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Botulinum Toxin Plantibodies

Award Information
Agency: Department of Defense
Branch: Office for Chemical and Biological Defense
Contract: DAMD17-03-C-0103
Agency Tracking Number: C031-0362
Amount: $69,808.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Solicitation Year: N/A
Award Year: 2003
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
25571 Clawiter Road
Hayward, CA 94545
United States
DUNS: 052917593
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Keith Wycoff
 Research Director
 (510) 887-1461
Business Contact
 Elliott Fineman
Title: Chief Executive Officer
Phone: (510) 887-1461
Research Institution

Our overall goal in this project is to make available inexpensive neutralizing antibodies against Botulinum neurotoxin (BoNT) in large quantity, using transgenic plants. Dr. James Marks (UC San Francisco School of Medicine) has identified murine and humansingle chain Fv (scFv) that bind non-overlapping epitopes on BoNT/A and neutralize toxin in vitro and in vivo. At present, plants offer the best system for the large scale, inexpensive production of these antibodies. Genes encoding the three bestantibodies, codon-optimized for plant expression, will be cloned into vectors designed for the expression of human IgG1 in leaves of transgenic tobacco and seed of Tepary bean (Phaseolus acutifolius). Plants will be transformed with these constructs,regenerated and screened for expression of antibody. Plants expressing high levels of the antibodies will be identified and used to purify enough of each antibody to characterize structure and binding affinity. We estimate that plants will facilitate theproduction of metric ton quantities of antibodies at 1-5% of the cost of steel tank bioreactors. Plantibody technology will greatly reduce the costs to stockpile antibodies for protection of the population from this serious threat. The successfulcompletion of the aims of this project will result in the availability of lage quantities of neutralizing anti-botulinum toxin antibodies at low cost. These antibodies would be used for passive immunization of people exposed to, or at risk of exposure tobotulinum toxin.

* Information listed above is at the time of submission. *

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