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USE OF A LYSOZYME OF BROAD SPECIFICITY TO PROTECT PLANTS AGAINST BACTERIAL PATHOGENS.

Award Information
Agency: National Science Foundation
Branch: N/A
Contract: N/A
Agency Tracking Number: 7592
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 1988
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
3330 Hillview Avenue
Palo Alto, CA 94304
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 ROSS ECCLESHALL
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract

BACTERIAL INVASION OF CROP PLANTS CAUSES ECONOMICALLY IMPORTANT DAMAGE. A COMMERCIAL IMPROVEMENT OF THESE PLANTS WOULD BE A GREATER RESISTANCE TO PHYTOPATHOGENIC BACTERIA. HENCE, THE OBJECTIVE OF THIS PROPOSAL IS TO EXPRESS A LYSOZYME OF HIGH SPECIFIC ACTIVITY IN A PLANT AND SHOW A BROAD SPECTRUM RESISTANCE TO PHYTOPATHOGENIC BACTERIA. THE LYSOZYME GENE OF FIRST CHOICE WILL BE THE HENEGG-WHITE LYSOZYME GENE BECAUSE THERE IS PUBLIC INFORMATION DESCRIBING THE ISOLATION AND SEQUENCING OF THE GENE. AN INTRONLESS VERSION OF THIS GENE HAS BEEN SHOWN TO BE EXPRESSIBLE IN YEAST. IN PHASE I, WE PROPOSE TO DETERMINE THE CONCENTRATIONS OF HEN EGG-WHITE LYSOZYME REQUIRED TO LYSE SEVERAL SPECIES OF PHYTOPATHOGENIC BACTERIA UNDER PHYSIOLOGICAL CONDITIONS. USING THAT INFORMATION, WE WILL DETERMINE IF EXOGENOUS LYSOZYME AT EFFECTIVE CONCENTRATIONS CAN PROTECT PLANTS FROM INFECTION. IF THE RESULTS INDICATE THAT HEN EGG-WHITE LYSOZYME COULD PROTECT AT A CONCENTRATIONWHICH COULD REASONABLY BE EXPECTED TO BE ACHIEVABLE THROUGH TRANSFORMATION AND EXPRESSION IN THE PLANT, THEN PHASE II WILL INVOLVE THE TRANSFORMATION OF A PLANT SPECIES TO STABLY INSERT THE LYSOZYME GENE INTO THE GENOME AND THE DEMONSTRATION OF INCREASED BACTERIAL RESISTANCE OF THE TRANSFORMED PLANTS.

* Information listed above is at the time of submission. *

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